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Purified anti-DDX1 Antibody

Pricing & Availability
Clone
9F12B04 (See other available formats)
Regulatory Status
RUO
Other Names
ATP-dependent RNA helicase DDX1, DEAD box protein retinoblastoma (DBP-RB)
Isotype
Mouse IgG1, κ
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Product Citations
publications
9F12B04_PURE_DDX1_Antibody_1_WB_071816
Whole cell lysates (15 µg protein) from HeLa and NIH/3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-DDX1 antibody, clone 9F12B04 . Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse-IgG secondary antibody (cat. No. 405306). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 9F12B04_PURE_DDX1_Antibody_1_WB_071816
    Whole cell lysates (15 µg protein) from HeLa and NIH/3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-DDX1 antibody, clone 9F12B04 . Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse-IgG secondary antibody (cat. No. 405306). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 9F12B04_PURE_DDX1_Antibody_2_IF_083016.
    HeLa cells were fixed with 2% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL purified anti-DDX1 antibody (clone 9F12B04) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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675302 100 µg 288 CHF
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Description

DDX1, also known as DEAD box protein retinoblastoma, is a member of the DEAD (Asp-Glu-Ala-Asp)-box RNA helicase family. DDX1 possesses RNA nuclease, binding, unwinding, and ATPase activities, and is thus involved in RNA metabolism, pre-mRNA splicing, translation initiation, and ribosome biogenesis. DDX1 interacts with the Rev protein of HIV-1 and has been shown to be crucial in the viral RNA replication. DDX1 was found to locate to the area of DNA damage induced by ionizing radiation and is implicated to participate in double-strand DNA break repair. DDX1 also acts as a transcriptional coactivator to facilitate NF-κB mediated gene activation. Elevated expression of DDX1 was observed in a number of tumors such as retinoblastoma, neuroblastoma, and testicular carcinoma. The accumulation of DDX1 is associated with cancer metastasis and poor survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DDX1 recombinant protein (1-107 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565509 (BioLegend Cat. No. 675302)

Antigen Details

Structure
740 amino acids with a predicted molecular weight of 82 kD. It contains a DEAD box motif and an ATP-binding helicase domain.
Distribution

Nucleus and cytoplasm.

Function
DDX1 is an ATP-dependent RNA helicase and possesses RNA nuclease activity and ATPase activity. DDX1 has been shown to participate in mRNA processing, DNA repair, and transcriptional activation.
Interaction
PHF5A, MBNL1, CSTF2, HNRNPK, ATM, and NFκB-p65.
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors
Antigen References

1. Lin MH, et al. 2014. Retrovirology 11:121.
2. Han C, et al. 2014. Cell Rep. 8:1447.
3. Edgcomb SP, et al. 2012. J. Mol. Biol. 415:61.
4. Taunk NK, et al. 2012. Cancer 118:888.
5. Xu L, et al. 2010. J. Virol. 84:8571.
6. Godbout R, et al. 2007. Future Oncol. 3:575.

Gene ID
1653 View all products for this Gene ID
UniProt
View information about DDX1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11.28.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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