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Purified anti-DNMT1 Antibody

Pricing & Availability
Clone
W15077A (See other available formats)
Regulatory Status
RUO
Other Names
DNA (cytosine-5-)-methyltransferase 1, CXXC9, MCMT, AIM, DNMT, HSN1E, ADACADN, m. Hasl
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15077A_PURE_DNMT1_Antibody_1_WB_082316
Total cell lysates (15 µg protein) from mouse brain, NTERA-2, Jurkat and HEL were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-DNMT1 Antibody (clone W15077A) (upper). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. α-Tubulin (lower) was used as a loading control.
  • W15077A_PURE_DNMT1_Antibody_1_WB_082316
    Total cell lysates (15 µg protein) from mouse brain, NTERA-2, Jurkat and HEL were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-DNMT1 Antibody (clone W15077A) (upper). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. α-Tubulin (lower) was used as a loading control.
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687502 100 µg 323 CHF
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Description

DNA (Cytisube-5-)-Methyltransferase 1 (DNMT-1), is an enzyme that has a role in the establishment and regulation of tissue-specific patterns of  methylated cytosine residues. This enzyme associates with DNA replication sites in the S phase and methylates CpG residues to maintain the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. In addition to this, it associates with DNA during the G2 and M phases to maintain DNA methylation. Diseases associated with abnormal DNMT-1 function include certain human tumors and developmental abnormalities. There are two transcript encoding different isoforms have been found for this gene.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DNMT1 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616944 (BioLegend Cat. No. 687502)

Antigen Details

Structure
1616 amino acids with a predicted molecular weight of 183.2 kD. Contains a C-terminal catalytic domain responsible for DNA methylation.
Distribution
Nuclear.
Function
DNMT1 is a DNA methyltransferase that is responsible for the establishment and maintaining DNA methylation patterns.
Interaction
Forms a homodimer. Interacts with CSNK1D, HDAC1, PCNA, DMAP1, HDAC2, MBD2, MBD3, UBC9, UHRF1, and USP7.
Biology Area
Cell Biology, Neuroscience, Transcription Factors
Antigen References

1. Merry CR, et al. 2015. Hum. Mol. Genet. 24:6240.
2. Cheng J, et al. 2015. Nat. Commun. 6:7023.
3. Pathania R, et al. 2015. Nat. Commun. 6:6910.
4. Liao J, et al. 2015. Nat. Genet. 47:469.
5. Espada J. 2012. Epigenetics 7:115.
6. Svedružić ŽM. 2011. Prog. Mol. Biol. Transl. Sci. 101:221.

Gene ID
1786 View all products for this Gene ID
UniProt
View information about DNMT1 on UniProt.org
Go To Top Version: 2    Revision Date: 08.23.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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