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Purified anti-DNMT3B Antibody

Pricing & Availability
Clone
W15184A (See other available formats)
Regulatory Status
RUO
Other Names
DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase 3 beta, DNA methyltransferase HsaIIIB
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W15184A_PURE_DNMT3B_Antibody_WB_021417
Total protein from U87-MG, NTERA2 and NF1 cell lysates (15µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-DNMT3B antibody (clone W15184A) or competitor's antibody used at manufactures recommended concentration. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • W15184A_PURE_DNMT3B_Antibody_WB_021417
    Total protein from U87-MG, NTERA2 and NF1 cell lysates (15µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-DNMT3B antibody (clone W15184A) or competitor's antibody used at manufactures recommended concentration. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
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695202 100 µg 276 CHF
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Description

DNA (cytosine-5-)-methyltransferase 3 beta, also known as DNMT3B, is an enzyme responsible for establishing DNA methylation patterns. DNA methyltransferases in mammals are comprised of four family members: DNMT1, DNMT3A, DNMT3B, and DNMT3L. DNMT3B, as well as DNMT3A, act as de novo methyltransferases that modify unmethylated DNA, whereas DNMT1 functions in the maintenance of DNA methylation pattern. DNA methylation is a covalent modification that involves the transfer of a methyl group from S-adenosylmethionine to the 5’ cytosine residue at CpG dinucleotides of the genomic DNA. CpG methylation is usually associated with transcriptional repression and is required for embryonic development, imprinting, genomic stability, and X-chromosome inactivation. Defects in DNMT3B gene in human cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DNMT3B recombinant protein (1-355 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Based on sequence homology, this antibody should recognize isoforms 1-6 of DNMT3B with MW ranging from 81kD to 96kD. However, according to the immunogen sequence, there is a possibility that the antibody might not recognize isoforms 7 and 8.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2650802 (BioLegend Cat. No. 695202)

Antigen Details

Structure
853 amino acids (isoform 1) with a predicted molecular weight of 96 kD. Contains two zinc finger domains and a SAM-dependent methyltransferase domain.
Distribution

Nucleus

Function
DNMT3B is a DNA methyltransferase that is responsible for the establishment of DNA methylation patterns.
Interaction
Interacts with BAZ2A/TIP5, SUV39H1, CBX4, UHRF1, DNMT1, DNMT3A, SETDB1, UBL1, UBE2I9, ZHX1, and the PRC2/EED-EZH2 complex.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience
Antigen References

1. Plourde KV, et al. 2016. Epigenomics. 8:1209.
2. Duymich CE, et al. 2016. Nat. Commun. 7:11453.
3. Manoharan A, et al. 2015. Eur. J. Immunol. 45:2343.
4. Liao J, et al. 2015. Nat. Genet. 47:469.
5. Baubec T, et al. 2015. Nature. 520:243.
6. Pawlak M, et al. 2011. Genes Dev. 25:1035.

Gene ID
1789 View all products for this Gene ID
UniProt
View information about DNMT3B on UniProt.org
Go To Top Version: 1    Revision Date: 02.15.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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