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Purified anti-E. coli RNA Polymerase α Antibody

Pricing & Availability
Clone
4RA2 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase subunit alpha, Transcriptase subunit alpha, pez, phs, sez
Isotype
Mouse IgG1
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Product Citations
publications
4RA2_PURE_RNAPolymerasealpha_WB_102717
Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase α antibody (clone 4RA2). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Lane M: Molecular weight ladder.
  • 4RA2_PURE_RNAPolymerasealpha_WB_102717
    Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase α antibody (clone 4RA2). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Lane M: Molecular weight ladder.
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663104 100 µg 323 CHF
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Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 ug/mL (1:500-1:5000). It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Lesley SA and Burgess RR. 1989. Biochemistry 28:7728.
Product Citations
  1. Sanderlin AG, et al. 2022. J Bacteriol. 204:e0018222. PubMed
  2. Prentice JA, et al. 2022. J Bacteriol. 204:e0024922. PubMed
  3. Liang Q, et al. 2022. Front Cell Infect Microbiol. 12:1081614. PubMed
  4. Park SY, et al. 2022. Cell. 185:513. PubMed
  5. Chang S, et al. 2022. Nat Struct Mol Biol. 29:932. PubMed
  6. Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed
  7. Hummels KR, et al. 2023. Nature. 615:300. PubMed
  8. Di Venanzio G, et al. 2019. Nat Commun. 10:2763. PubMed
  9. Hernandez–Doria JD, et al. 2018. Cell Host Microbe. 23:607. PubMed
  10. Shin JH, et al. 2021. EMBO Rep. 22:e51790. PubMed
  11. Tomasek D, et al. 2020. Nature. 583:473. PubMed
  12. Han K, et al. 2021. MBio. 12:. PubMed
  13. Shin JH, et al. 2020. Biochem Biophys Res Commun. 528:493. PubMed
  14. Martin NR, et al. 2021. Nat Microbiol. 6:910. PubMed
  15. Weaver AI, et al. 2022. Elife. 11:. PubMed
  16. Guo MS et al. 2018. Cell. 175(2):583-597 . PubMed
  17. Zingl FG, et al. 2020. Cell Host & Microbe. 27(2):225-237.e8.. PubMed
  18. Bridges AA, et al. 2021. Elife. 10:. PubMed
  19. Yang N, et al. 2020. Mol Microbiol. 114:423. PubMed
  20. Guzzo M, et al. 2021. Dev Cell. 56:2145. PubMed
RRID
AB_2687386 (BioLegend Cat. No. 663104)

Antigen Details

Structure
α is a 36 kD subunit of the E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947794 View all products for this Gene ID
UniProt
View information about RNA Polymerase alpha on UniProt.org
Go To Top Version: 2    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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