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Purified anti-E1 Ubiquitin Activating Enzyme Antibody

Pricing & Availability
Clone
2G2.3.5 (See other available formats)
Regulatory Status
RUO
Other Names
Ubiquitin-Activating Enzyme E1, Ubiquitin-Like Modifier Activating Enzyme 1, UBE1, A1S9T, GXP1, SMAX2, 2G.2.3.5, 2G2.3-5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-2G2-3-5_Purified_E1_Ubiquitin_Activating_Enzyme_Antibody_1_WB_032619
Total cell lysates (15 µg total protein) from HeLa and HepG2 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.25 µg/mL Purified anti-E1 Ubiquitin Activating Enzyme Antibody, clone 2G2.3.5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • a-2G2-3-5_Purified_E1_Ubiquitin_Activating_Enzyme_Antibody_1_WB_032619
    Total cell lysates (15 µg total protein) from HeLa and HepG2 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.25 µg/mL Purified anti-E1 Ubiquitin Activating Enzyme Antibody, clone 2G2.3.5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b-2G2.3.5_Purified_E1_Ubiquitin_Activating_Enzyme_Antibody_2_IF_050115
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Cells were then intracellularly stained with 2 µg/ml of anti-E1 Ubiquitin Activating Enzyme antibody (clone 2G2.3.5 ) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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932002 100 µg 311 CHF
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Description

The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixture of human and mouse E1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.
RRID
AB_2565603 (BioLegend Cat. No. 932002)

Antigen Details

Structure
1058 amino acids with a predicted molecular weight of approximately 118 kD.
Distribution

Ubiquitous, Eukaryotic cells.

Function
This protein catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation.
Interaction
GAN.
Biology Area
Cell Biology, DNA Repair/Replication, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Protein Modifiers
Antigen References

1. Xu W, et al. 2013. Curr. Pharm. Des. 18:3201.
2. Neutzner M, et al. 2012. Essays Biochem. 52:37.
3. Schulman BA, et al. 2009. Nat. Rev. Mol. Cell Biol. 10:319.
4. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.

Gene ID
7317 View all products for this Gene ID
UniProt
View information about E1 Ubiquitin Activating Enzyme on UniProt.org
Go To Top Version: 4    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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