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Purified anti-EAAT2 Antibody

Pricing & Availability
Clone
A18128E (See other available formats)
Regulatory Status
RUO
Other Names
GLT1, SLC1A2, Excitotoxic Amino Acid Transporter 2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18128E_PURE_EAAT2_Antibody_082019
Western blot of purified anti-EAAT2 antibody (clone A18128E). Lane 1: Molecular weight marker; Lane 2: 20 ug of human brain lysate; Lane 3: 20 ug of mouse brain lysate; Lane 4: 20 ug of rat brain lysate. The blot was incubated with 2 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18128E_PURE_EAAT2_Antibody_082019
    Western blot of purified anti-EAAT2 antibody (clone A18128E). Lane 1: Molecular weight marker; Lane 2: 20 ug of human brain lysate; Lane 3: 20 ug of mouse brain lysate; Lane 4: 20 ug of rat brain lysate. The blot was incubated with 2 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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869201 25 µg 106 CHF
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869202 100 µg 276 CHF
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Description

EAAT2 is the major membrane protein that clears extracellular glutamate from the synaptic area to prevent potential neuronal excitotoxicity from excessive activation of glutamate receptors. EAAT2 is predominantly expressed in the central nervous system on astrocytes and is frequently used as a marker for these cells. Mutations that affect EAAT2 function are clinically associated with amyotrophic lateral sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant mouse SLC1A2 (EAAT2) protein (a.a. 143 - 238) with GST Tag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814631 (BioLegend Cat. No. 869201)
AB_2814631 (BioLegend Cat. No. 869202)

Antigen Details

Structure
EAAT2 is a 574 amino acid protein with predicted and observed molecular mass of ~ 62 kD.
Distribution

Tissue Distribution: Brain

Cellular Distribution: Plasma membrane

Function
EAAT2 is a sodium-dependent amino acid transporter that mediates the uptake of L-Glutamate and L- or D- aspartate.
Interaction
EAAT2 forms homotrimer and interacts with JUB.
Cell Type
Astrocytes
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Pregnolato S, et al. 2019. Front Physiol. 10:417
  2. Parkin GM, et al. 2018. World J Psychiatry. 10.5498/wjp.v8.i2.51 
  3. Rosenblum LT, Trotti D. 2017. Adv. Neurobiol. 16:117-136. 
Gene ID
6506 View all products for this Gene ID 20511 View all products for this Gene ID 29482 View all products for this Gene ID
UniProt
View information about EAAT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.20.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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