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Purified anti-EED Antibody

Pricing & Availability
Clone
W18151A (See other available formats)
Regulatory Status
RUO
Other Names
Embryonic Ectoderm Development, Embryonic Ectoderm Development Protein, Polycomb Protein EED
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18151A_PURE_EED_Antibody_1_112719_updated
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-EED antibody (clone W18151A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18151A_PURE_EED_Antibody_1_112719_updated
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-EED antibody (clone W18151A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18151A_PURE_EED_Antibody_2_112719_updated
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-EED antibody (clone W18151A). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL of purified anti-EED antibody (clone W18151A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-rat light chain specific antibody. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18151A_PURE_EED_Antibody_3_112719_updated
    Whole cell extracts (15 µg protein) from HeLa cells transfected with scramble control siRNA or siRNA targeting EED were resolved on a 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL of purified anti-EED antibody (clone W18151A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. siRNA targeting EED for depletion was obtained from Dharmcon (SMARTpool ON-TARGETplus EED siRNA, Cat. No. L-017581-00-0005)
  • W18151A_PURE_EED_Antibody_4_112619
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1 µg/mL of either purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502, panel A) or purified anti-EED antibody (clone W18151A, panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
934901 25 µg 101 CHF
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934902 100 µg 241 CHF
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Description

Embryonic ectoderm development (EED) is a member of the polycomb-group (PcG) family. The PcG family members form multimeric protein complexes called polycomb repressive complexes (PRCs). These polycomb repressive complexes (PRCs) epigenetically silence gene expression and are involved in a variety of biological processes such as cell cycle control, stem cell plasticity, cellular senescence and cancer.

There are multiple isoforms (~4), which seem to interact differently with different PRCs. Isoform 1 of EED also has an alternate phosphorylated form. Diseases associated with EED include Cohen-Gibson syndrome and Weaver syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human EED protein of approximately 250 amino acids
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes 3 isoforms of human and mouse EED.

For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg/mL.

RRID
AB_2820221 (BioLegend Cat. No. 934901)
AB_2820221 (BioLegend Cat. No. 934902)

Antigen Details

Structure
EED is an ~440 amino acid protein with 4 isoforms of a predicted molecular weight of around 50 kD.
Distribution

EED mainly localizes to the nucleoplasm.

Function
Maintains transcriptionally repressive state of genes.
Interaction
EZH2
Biology Area
Cancer Biomarkers, Cell Cycle/DNA Replication, Cell Death, Chromatin Remodeling/Epigenetics, Stem Cells
Antigen References

Van der Vlag J, et al. 1999. Nat. Gen. 23:474.

Martin C, et al. 2006. J. Bol. 281:13:8365.

Cao Q, et al. 2014. Nat. Comm.  5:3127.

Kuzmichev A, et al. 2004. Mol. Cell. 14:183.

Vire E, et al. 2005. Nat. 439:871.

Schuettengruber B, et al. 2009. Development.  136: 353.

Gene ID
8726 View all products for this Gene ID
UniProt
View information about EED on UniProt.org
Go To Top Version: 1    Revision Date: 11.26.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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