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Purified anti-Eg5 Antibody

Pricing & Availability
Clone
10C7/Eg5 (See other available formats)
Regulatory Status
RUO
Other Names
Kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, thyroid receptor interacting protein 5 (TRIP5)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10C7-EG5_Purified_Eg5_Antibody_1_112818
Total cell lysates (15 µg total protein) from A431, SH-SY5Y, MCF-7 (reduced expression control), and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) Purified anti-EG5 Antibody, clone 10C7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 10C7-EG5_Purified_Eg5_Antibody_1_112818
    Total cell lysates (15 µg total protein) from A431, SH-SY5Y, MCF-7 (reduced expression control), and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) Purified anti-EG5 Antibody, clone 10C7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
Cat # Size Price Quantity Check Availability Save
627801 25 µg 106 CHF
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627802 100 µg 229 CHF
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Description

Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2. The 10C7/Eg5 monoclonal antibody has been shown to react with human and mouse Eg5 by Western blot.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino Acid: 200-500 of human Eg5
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
  2. Irelan JT, et al. 2007. P. Natl. Acad. Sci. USA 104:16940.
  3. Bartoli, KM., et al. 2011. Mol Cell Biol. 22:3420. PubMed
  4. Izumiyama T, et al. 2012. Gene. 511:202. PubMed
Product Citations
  1. Gury-BenAri M, et al. 2016. Cell. 1231:166. PubMed
  2. Bartoli K, et al. 2011. Mol Biol Cell. 3.291666667. PubMed
  3. Fang CT, et al. 2020. Cell Death Dis. 0.954861111. PubMed
  4. Izumiyama T, et al. 2012. Gene. 511:202. PubMed
RRID
AB_2130963 (BioLegend Cat. No. 627801)
AB_2130963 (BioLegend Cat. No. 627802)

Antigen Details

Structure
Kinesin-like protein family, BimC subfamily, kinesin motor catalytic, coiled-coil, Smc domains; 119 kD
Distribution

Centrosomes, spindle microtubules, intracellular bridge

Function
Motor protein required for establishing bipolar spindle
Interaction
Thyroid hormone receptor in presence of thyroid hormone, Cdc2
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Lee J, et al. 1995. Mol. Endocrinol. 9:243.
2. Blangy A, et al. 1995. Cell 83:1159.
3. Whitehead C, et al. 1998. J. Cell Sci. 111:2551.
4. DeBonis S, et al. 2003. Biochemistry. 42:338.

Regulation
Phosphorylation on Thr927 by Cdc2 allows association with spindle apparatus
Gene ID
3832 View all products for this Gene ID
UniProt
View information about Eg5 on UniProt.org
Go To Top Version: 2    Revision Date: 11.28.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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