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Purified anti-EGFR Phospho (Tyr1148) Antibody

Pricing & Availability
Clone
Poly21A02 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly21A02_PURE_EGFR-Phospho-Tyr1148_Antibody_1_120524
Whole cell extracts (15 µg protein) from A-431 cells either untreated (-) or treated (+) with recombinant human EGF (Cat. No. 585506) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:2000 dilution of purified anti-EGFR Phospho (Tyr1148) (clone Poly21A02) for two hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Total EGFR loading was confirmed using purified anti-EGFR (clone A19002A) (Cat. No. 933902) at 0.1 µg/mL. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • Poly21A02_PURE_EGFR-Phospho-Tyr1148_Antibody_1_120524
    Whole cell extracts (15 µg protein) from A-431 cells either untreated (-) or treated (+) with recombinant human EGF (Cat. No. 585506) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:2000 dilution of purified anti-EGFR Phospho (Tyr1148) (clone Poly21A02) for two hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Total EGFR loading was confirmed using purified anti-EGFR (clone A19002A) (Cat. No. 933902) at 0.1 µg/mL. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • Poly21A02_PURE_EGFR-Phospho-Tyr1173_Antibody_2_022422
    ICC staining of purified anti-EGFR Phospho (Tyr1148) (clone Poly21A02) on serum-starved A-431 cells untreated (negative control) (panel A) or treated with recombinant human EGF (positive control) (panel B) (Cat. No. 585506). The cells were fixed with 4% PFA and permeabilized with 100% methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with a 1:40 dilution of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 donkey anti-rabbit IgG (Cat. No. 406418) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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602151 50 µL 293 CHF
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602152 200 µL 692 CHF
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Description

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. EGFR undergoes autophoshorylation at tyrosine 1148, which leads to the recruitment of SH-2 domain-containing proteins SHC and Grb2 and concomitant downstream activation of ERK. In addition, EGFR stimulation can activate PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide corresponding to the mature form of human EGFR phosphorylated at tyrosine 1148
Formulation
Phosphate-buffered solution containing 0.09% sodium azide with 50% glycerol in an aqueous buffered solution
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted at -20°C
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:2000. For immunocytochemistry, a dilution of 1:40 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For Western blot, we recommend using Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) for detection, and 2 hour incubation times with Poly21A02.

RRID
AB_2924608 (BioLegend Cat. No. 602151)
AB_2924608 (BioLegend Cat. No. 602152)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Ligand/Receptor
EGF
Cell Type
Epithelial cells
Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Hunter T and Cooper JA. 1981. Cell. 24:741-52.
  2. Gill GN and Lazar CS. 1981. Nature. 293:305-7.
  3. Reynolds FH, et al. 1981. Nature. 292:259-62.
  4. Biscardi JS, et al. 1999. J Biol Chem. 274:8335-43.
  5. Zhou M, et al. 2013. Cancer Res. 23:7056-67.
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.03.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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