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Purified anti-EGFR Phosphorylated (Tyr1068) Antibody

Pricing & Availability
Clone
Poly6563 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal growth factor receptor, HER1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6563_Purified_EGFR_Antibody_WB_071813
Hela cells were starved for 16 hours and treated with (lane 2) or without (lane 1) 100 ng/mL EGF and 1 mM pervanadate for 15 minutes. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-EGFR (Tyr1068) antibody. Proteins were visualized using an anti-Rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
  • Poly6563_Purified_EGFR_Antibody_WB_071813
    Hela cells were starved for 16 hours and treated with (lane 2) or without (lane 1) 100 ng/mL EGF and 1 mM pervanadate for 15 minutes. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-EGFR (Tyr1068) antibody. Proteins were visualized using an anti-Rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
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656302 200 µL 411 CHF
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Description

EGFR is a transmembrane glycoprotein that belongs to protein kinase superfamily. It is expressed in tissues of epithelial, mesenchymal and neuronal origin and plays major roles in proliferation, differentiation and development. Binding of the protein to its ligand induces receptor dimerization and tyrosine autophosphorylation. Mutations or over-expression of this gene are associated with certain forms of cancer. Tyrosine 1068 is the primary autophosphorylation site in the C-terminal domain of the receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
KLH conjugated modified synthetic peptide
Formulation
Phosphate-buffered solution/50% glycerol/0.09% sodium azide.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store undiluted at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5-10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

RRID
AB_2562283 (BioLegend Cat. No. 656302)

Antigen Details

Structure
Four isoforms with 405 to 1210 amino acids, predicted molecular weight 45- 170 kD
Distribution

Cell membrane, nuclear membrane and cellular organelle membrane

Interaction
Interacts with EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Activates RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules.
Biology Area
Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Alameddine RS, et al. 2013. Curr. Opin. Oncol. 25:313.
2. Downward J, et al. 2003. Anticancer Res. 23:3639.
3. Hrustanovic G, et al. 2013. Cancer Biol. Ther. 14:304.
4. Koland JG, et al. 1990. Biochem. Biophys. Res. Commun. 166:90.
5. Wheeler SE, et al. 2012. Clin. Cancer Res. 18:2278.
6. Yano S, et al. 2003. Anticancer Res. 23:3639.

Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org
Go To Top Version: 3    Revision Date: 03.17.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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