- Clone
- A17195G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PKR, PRKR, E2AK2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
871101 | 25 µg | 106 CHF | ||||
871102 | 100 µg | 276 CHF |
IFN-induced dsRNA-dependent serine/threonine-protein kinase (EIF2AK2) is an important component of the innate immune response to viral infection. The N-terminal of EIF2AK2 contains a double stranded RNA (dsRNA) binding domain, which binds dsRNA introduced to the host cells by viral infection, and activates a pro-apoptotic signal in the host cell. Recent studies have shown that activation of EIF2AK2 can lead to the accumulation of amyloid beta in Alzheimer disease patients, suggesting it plays a role in neurodegenerative disorders.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant human EIF2AK2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody was purified by affinity chromatography.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody reacts stronger with rat tissue samples than human cell line samples. Recommended WB usage for rat brain: 0.5 - 2 µg/mL; Recommended WB usage for human cell lines: 2 - 10 µg/mL
- RRID
-
AB_2832878 (BioLegend Cat. No. 871101)
AB_2832878 (BioLegend Cat. No. 871102)
Antigen Details
- Structure
- Human EIF2AK2 has 2 isoforms. Isoform 1 is an 551 amino acid protein with predicted and observed molecular mass of ~62 kD; Isoform 2 is an 510 amino acid protein with predicted and observed molecular mass of ~57 kD
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus, Cytosol - Function
- Innate immune response against viral infection, central to stress signals
- Interaction
- Forms homodimer, interacts with DNAJC3, STRBP, ADAR
- Ligand/Receptor
- dsRNA
- Cell Type
- Astrocytes, Endothelial cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- APP/β-Amyloid, Innate Immune Signaling, Protein Kinases/Phosphatase
- Antigen References
-
- Chesnokova E, et al. 2017. Int J Mol Sci. 18(10).
- Bou-Nader C, et al. 2019. RNA. 25(5):539.
- Dzananovic E, et al. 2018. Biotechnol Genet Eng Rev. 34(1):33.
- Gene ID
- 5610 View all products for this Gene ID 54287 View all products for this Gene ID
- UniProt
- View information about EIF2AK2 on UniProt.org
Other Formats
View All EIF2AK2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-EIF2AK2 | A17195G | WB,IHC-P,ICC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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