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Purified anti-EIF2AK2 Antibody

Pricing & Availability
Clone
A17195G (See other available formats)
Regulatory Status
RUO
Other Names
PKR, PRKR, E2AK2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17195G_PURE_EIF2AK2_Antibody_1_021920.png
Western blot of purified anti-EIF2AK2 antibody (clone A17195G). Lane 1: Molecular weight marker; Lane 2: 30 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17195G_PURE_EIF2AK2_Antibody_1_021920.png
    Western blot of purified anti-EIF2AK2 antibody (clone A17195G). Lane 1: Molecular weight marker; Lane 2: 30 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17195G_PURE_EIF2AK2_Antibody_2_021920.png
    Western blot of purified anti-EIF2AK2 antibody (clone A17195G). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17195G_PURE_EIF2AK2_Antibody_3_021920.png
    IHC staining of purified anti-EIF2AK2 antibody (clone A17195G) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17195G_PURE_EIF2AK2_Antibody_4_021920.png
    ICC staining of purified anti-EIF2AK2 antibody (clone A17195G) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. Flash Phalloidin™ Green 488 (Cat. No. 424201) was used to stain the actin cytoskeleton. The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability Save
871101 25 µg 106 CHF
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871102 100 µg 276 CHF
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Description

IFN-induced dsRNA-dependent serine/threonine-protein kinase (EIF2AK2) is an important component of the innate immune response to viral infection. The N-terminal of EIF2AK2 contains a double stranded RNA (dsRNA) binding domain, which binds dsRNA introduced to the host cells by viral infection, and activates a pro-apoptotic signal in the host cell. Recent studies have shown that activation of EIF2AK2 can lead to the accumulation of amyloid beta in Alzheimer disease patients, suggesting it plays a role in neurodegenerative disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human EIF2AK2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody was purified by affinity chromatography.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts stronger with rat tissue samples than human cell line samples. Recommended WB usage for rat brain: 0.5 - 2 µg/mL; Recommended WB usage for human cell lines: 2 - 10 µg/mL

RRID
AB_2832878 (BioLegend Cat. No. 871101)
AB_2832878 (BioLegend Cat. No. 871102)

Antigen Details

Structure
Human EIF2AK2 has 2 isoforms. Isoform 1 is an 551 amino acid protein with predicted and observed molecular mass of ~62 kD; Isoform 2 is an 510 amino acid protein with predicted and observed molecular mass of ~57 kD
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus, Cytosol

Function
Innate immune response against viral infection, central to stress signals
Interaction
Forms homodimer, interacts with DNAJC3, STRBP, ADAR
Ligand/Receptor
dsRNA
Cell Type
Astrocytes, Endothelial cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
APP/β-Amyloid, Innate Immune Signaling, Protein Kinases/Phosphatase
Antigen References
  1. Chesnokova E, et al. 2017. Int J Mol Sci. 18(10).
  2. Bou-Nader C, et al. 2019. RNA. 25(5):539.
  3. Dzananovic E, et al. 2018. Biotechnol Genet Eng Rev. 34(1):33.
Gene ID
5610 View all products for this Gene ID 54287 View all products for this Gene ID
UniProt
View information about EIF2AK2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.19.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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