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Purified anti-Elongin B Antibody

Pricing & Availability
Clone
10C4 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase II transcription factor SIII p18 subunit, transcription elongation factor B polypeptide 2, TCEB2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10C4_PURE_ElonginB_Antibody_WB_062618
Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1), 1 µg/mL (1:500 dilution, lane 2) and 2 µg/mL (1:250 dilution, lane 3) purified anti-Elongin B antibody, clone 10C4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Elongin B antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 10C4_PURE_ElonginB_Antibody_WB_062618
    Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1), 1 µg/mL (1:500 dilution, lane 2) and 2 µg/mL (1:250 dilution, lane 3) purified anti-Elongin B antibody, clone 10C4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Elongin B antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
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629302 100 µg 229 CHF
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Description

Elongin B (also known as RNA polymerase II transcription factor SIII p18 subunit and transcription elongation factor B polypeptide 2) is a member of the UbH family containing a ubiquitin-like domain. Elongin functions as a subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. Clone 10C4 recognizes the human elongin B protein and is useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sato Y, et al. 2009. PLoS One. 5:e1000530. PubMed
RRID
AB_2201267 (BioLegend Cat. No. 629302)

Antigen Details

Structure
UbH family, ubiquitin-like domain, heterotrimer; Human Elongin B has two isoforms with predicted MW of 13kD and 18kD
Distribution

Nuclear

Function
Regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites
Interaction
Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Duan D, et al. 1995. Science. 269:1402.
2. Stebbins C, et al. 1999. Science. 284:455.
3. Brower C, et al. 2002. P. Natl. Acad. Sci. USA 99:10353.
4. DeRenzo C, et al. 2003. Nature. 424:685.

Gene ID
6923 View all products for this Gene ID
UniProt
View information about Elongin B on UniProt.org
Go To Top Version: 3    Revision Date: 06.26.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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