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Purified anti-EML4 Antibody

Pricing & Availability
Clone
W18139A (See other available formats)
Regulatory Status
RUO
Other Names
EML4, Echinoderm microtubule-associated protein-like 4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W18139A_PURE_EML4_Antibody_1_091019.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-EML4 Antibody, clone W18139A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Cell lines were loaded in order of increasing predicted EML4 expression (source: Human Protein Atlas).
  • W18139A_PURE_EML4_Antibody_1_091019.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-EML4 Antibody, clone W18139A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Cell lines were loaded in order of increasing predicted EML4 expression (source: Human Protein Atlas).
  • W18139A_PURE_EML4_Antibody_2_091019.png
    HepG2 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold Methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of either Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602, panel A) or Purified anti-EML4 Antibody, clone W18139A (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
670801 25 µg 101 CHF
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670802 100 µg 253 CHF
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Description

Echinoderm microtubule-associated protein-like 4 or EML4 plays a central role in the modification of microtubule structural dynamics and morphology. During mitosis, EML4 expression is upregulated and is associated with spindle formation. In lung cancer cells, a translocation mutation is associated with the EML4 gene, where parts of the gene fuses with the anaplastic lymphoma receptor tyrosine kinase (ALK) gene and generates an EML4-ALK fusion protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human EML4 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg/mL. For immunocytochemistry, a concentration of 1.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for IP.

RRID
AB_2814524 (BioLegend Cat. No. 670801)
AB_2814524 (BioLegend Cat. No. 670802)

Antigen Details

Structure
EML4 is a 981 amino acid protein with a predicted molecular weight of 109 kD
Distribution

Cytoskeleton/Ubiquitously expressed

Function
Microtubule formation
Antigen References
  1. Yoshida A, et al. 2011. J Thorac Oncol. 10:1677-86.
  2. Soda M, et al. 2007. Nature. 448:561.
  3. Pollmann M, et al. 2006. Exp Cell Res. (17):3241-51.

 

Gene ID
27436 View all products for this Gene ID
UniProt
View information about EML4 on UniProt.org
Go To Top Version: 1    Revision Date: 09.10.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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