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Purified anti-EOS Antibody

Pricing & Availability
Clone
W15169A (See other available formats)
Regulatory Status
RUO
Other Names
Ikaros family zinc finger protein 4 (IKZF4), KIAA1782, ZNFN1A4
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15169A_PURE_EOS_Antibody_071817
Total protein from HeLa (negative control cell line), K562 and NIH3T3 cell lysates (15 µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-EOS antibody (clone W15169A, upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (lower).
  • W15169A_PURE_EOS_Antibody_071817
    Total protein from HeLa (negative control cell line), K562 and NIH3T3 cell lysates (15 µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-EOS antibody (clone W15169A, upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (lower).
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695302 100 µg 288 CHF
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Description

EOS, also known as IKZF4 (Ikaros family zinc finger 4), is a member of the Ikaros family of zinc finger transcription factors, which are key transcriptional repressors during development of lymphocytes. Each of the five Ikaros family members (Ikaros, Helios, Aiolos, Eos and Pegasus) has N-terminal and C-terminal zinc-finger motifs responsible for DNA binding and dimerization, respectively. EOS is a highly conserved DNA-binding protein that recognizes the typical 5'WGGAAT-3' Ikaros-binding sequence. EOS has been implicated in the development of lymphoid cells and nervous systems, as well as mediating FOXP3-dependent gene silencing in CD4+ regulatory T cells though interacting with FOXP3 and CTBP1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human EOS recombinant protein (271-520 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 - 1:250 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W15169A does not cross-react with mouse (in-house tested). This antibody recognizes both isoforms of EOS and it also detects a non-specific protein of approximately 75 kD in the K562 cell line tested.

RRID
AB_2715762 (BioLegend Cat. No. 695302)

Antigen Details

Structure
EOS has two isoforms. Isoform 1 has 585 amino acids with a predicted molecular weight of 64.1 kD. Isoform 2 has 544 amino acids with a predicted molecular weight of 59.7 kD. Both isoforms contain six zinc finger domains responsible for DNA-binding or prot
Distribution

Nucleus

Function
EOS is a zinc finger transcription factor that is implicated in the development of lymphoid cells and nervous systems.
Interaction
EOS forms homodimers or heterodimers with other Ikaros family members. Interacts with CTBP2, SPI1, MITF, FOXP3, and CTBP1.
Antigen References

1. Rieder SA, et al. 2015. J. Immunol. 195:553.
2. Raffin C, et al. 2013. J. Immunol. 191:4619.
3. Paradzik M, et al. 2012. Int. J. Immunopathol. Pharmacol. 25:893.
4. Bettini ML, et al. 2012. Immunity. 36:717.
5. John LB, Ward AC. 2011. Mol. Immunol. 48:1272.
6. Pan F, et al. 2009. Science. 325:1142.

Gene ID
64375 View all products for this Gene ID
UniProt
View information about EOS on UniProt.org
Go To Top Version: 0    Revision Date: 07.19.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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