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Purified anti-ERK1/2 Antibody

Pricing & Availability
Clone
W15133B (See other available formats)
Regulatory Status
RUO
Other Names
Extracellular signal-regulated kinase 1/2, Mitogen-activated protein kinase MAPK3/MAPK1, p44ERK1/p42ERK2, p44-MAPK/p42MAPK, PRKM3/PRKM2, ERK
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
1_W15133B_PURE_ERK1slash2_Antibody_1_WB_071416_updated
Total lysates (15µg protein) from 293T, 293T/ERK1 knockout (KO) and 293T/ERK2 knockdown (KD) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-ERK1/2 antibody, clone W15133B (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-ERK1/2 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 1_W15133B_PURE_ERK1slash2_Antibody_1_WB_071416_updated
    Total lysates (15µg protein) from 293T, 293T/ERK1 knockout (KO) and 293T/ERK2 knockdown (KD) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-ERK1/2 antibody, clone W15133B (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-ERK1/2 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 2_W15133B_PURE_ERK1slash2_Antibody_2_WB_071416
    Total cell lysate from human HeLa cells (lane 1), Jurkat cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-ERK1/2 (clone W15133B) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection.
  • 3_W15133B_PURE_ERK1slash2_Antibody_2_ICC_041619
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.0 µg/ml purified anti-ERK1/2 (clone W15133B) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-rat IgG antibody (red) and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
  • 4_W15133B_PURE_ERK1slash2_Antibody_3_WB_082719
    Western blot of purified anti-ERK1/2 antibody (clone W15133B). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
686901 25 µg 112 CHF
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686902 100 µg 265 CHF
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Description

ERK1/2 are members of the mitogen-activated kinases (MAPKs) that are serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg/ml in human, mouse, and Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

The drosophila homolog of mammalian ERK1/2 is the 43 kD mitogen-activated protein kinase ERK-A.

Product Citations
  1. Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed
  2. McFall T, et al. 2021. Cell Rep. 37:110096. PubMed
  3. McFall T, et al. 2022. NPJ Precis Oncol. 6:86. PubMed
  4. Zhang Q, et al. 2021. Transl Res. 235:129. PubMed
  5. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  6. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
RRID
AB_2629535 (BioLegend Cat. No. 686901)
AB_2629535 (BioLegend Cat. No. 686902)

Antigen Details

Structure
ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution

Nucleus and cytoplasm.

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction
Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5595 View all products for this Gene ID 5594 View all products for this Gene ID
UniProt
View information about ERK1/2 on UniProt.org
Go To Top Version: 2    Revision Date: 07.15.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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