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Purified anti-Fatty Acid Synthase Antibody

Pricing & Availability
Clone
2F9-B2-B6 (See other available formats)
Regulatory Status
RUO
Other Names
FASN
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2F9-B2-B6_PURE_FattyAcidSynthase_Antibody_1_113021
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Fatty Acid Synthase (clone 2F9-B2-B6) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 2F9-B2-B6_PURE_FattyAcidSynthase_Antibody_1_113021
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Fatty Acid Synthase (clone 2F9-B2-B6) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 2F9-B2-B6_PURE_FattyAcidSynthase_Antibody_2_113021
    ICC staining of purified anti-Fatty Acid Synthase (clone 2F9-B2-B6) on HeLa cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 1.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • 2F9-B2-B6_PURE_FattyAcidSynthase_Antibody_3_113021
    IHC staining using purified anti-Fatty Acid Synthase (clone 2F9-B2-B6) on formalin-fixed paraffin-embedded human breast tissue. The tissue was incubated with 10 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 μM
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600151 25 µL 118 CHF
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600152 100 µL 293 CHF
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Description

Fatty acid synthase is a lipogenic enzyme required for the terminal step of de novo fatty acid synthesis. Using NAPDH as a cofactor, it catalyzes the synthesis of palmitate from acetyl- and malonyl-CoA precursors. Fatty acid synthase is upregulated and serves as a prognostic marker in multiple cancers. In contrast to normal tissue, where the enzyme functions in anabolic lipid storage pathways, fatty acid synthase supports tumor growth by providing intermediates for membrane biosynthesis and provides alternative fuel sources for ATP production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human fatty acid synthase protein
Formulation
0.5% BSA, 0.09% NaN3, bottle antibody at 0.2 mg/mL
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:2000. For immunocytochemistry, a dilution of 1:200 is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:20 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

During PD testing, additional bands below fatty acid synthase were observed. These were also produced by a control antibody raised with a different fatty acid synthase immunogen. We therefore believe these represent fatty acid synthase degradation products.

For ICC staining, we recommend using 4% PFA plus methanol or methanol only as fixation-permeabilzation methods. We do not recommend 4% PFA + Triton X-100 due to high non-specific nuclear staining when this method is used.

RRID
AB_2904436 (BioLegend Cat. No. 600151)
AB_2904436 (BioLegend Cat. No. 600152)

Antigen Details

Structure
Fatty acid synthase is a 2,511 amino acid protein with a predicted molecular weight of 273 kD.
Distribution

Cytosol

Function
Enzyme involved in fatty acid biosynthesis
Biology Area
Cell Biology, Mitochondrial Function
Antigen References
  1. Kuhajda FP, et al. 1994. Proc. Natl. Acad. Sci. USA. 91:6379-83.
  2. Menendez JA and Lupu R. 2007. Nat. Rev. Canc. 7:763-77.
  3. Jones SF and Infante JR. 2015. Clin. Cancer Res. 21:5434-8.
Gene ID
2194 View all products for this Gene ID
UniProt
View information about Fatty Acid Synthase on UniProt.org
Go To Top Version: 2    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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