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Purified anti-FGF-19 Antibody

Pricing & Availability
Clone
13D9C80 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast growth factor 19
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
13D9C80_PURE_FGF-19_Antibody_WB_100115
20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 13D9C80_PURE_FGF-19_Antibody_WB_100115
    20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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681102 100 µg 288 CHF
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Description

FGF-19 belongs to a subfamily of the fibroblast growth factors (FGFs) called endocrine FGFs, which consists of FGF-19, FGF-21, and FGF-23. Unlike other subfamily of FGFs, FGF-19 has a low binding affinity to heparin sulfate and functions as a circulating hormone with effects on distant tissues. FGF-19 has reduced binding affinity to the FGF receptors and the interaction requires two necessary cofactors that are α-Klotho and β-Klotho. In the ileum, FGF-19 expression is initiated by bile acids-induced activation of farnesoid X receptor (FXR). In response to bile acid stimulation, FGF-19 is secreted and enters the portal venous circulation, which subsequently down-regulates hepatic bile acid synthesis through repression of the CYP7A1 gene encoding cholesterol 7-alpha-monooxygenase. The impaired regulation of bile acids by FGF-19 leads to bile acid diarrhea also known as idiopathic bile acid malabsorption. FGF-19 has also been demonstrated to regulate carbohydrate and lipid metabolism.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human FGF-19 recombinant protein (25-216 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566443 (BioLegend Cat. No. 681102)

Antigen Details

Structure
Growth factor. 216 amino acids with a predicted molecular weight of 24 kD.
Distribution
Secreted.
Function
FGF-19 functions as a hormone, regulating homeostasis of bile acid, glucose, lipids, phosphate, and vitamin D.
Interaction
Interacts with heparian, α-Klotho, and β-Klotho.
Ligand/Receptor
FGFR1, FGFR2, FGFR3, and FGFR4.
Biology Area
Cell Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Xie MH, et al. 1999. Cytokine 11:729.
2. Harmer NJ, et al. 2004. Biochemistry 43:629.
3. Goetz R, et al. 2007. Mol. Cell Biol. 27:3417.
4. Wu X, et al. 2008. J. Biol. Chem. 283:33304.
5. Song KH, et al. 2009. Hepatology 49:297.
6. Turner N, et al. 2010. Nat. Rev. Cancer 10:116.

Gene ID
9965 View all products for this Gene ID
UniProt
View information about FGF-19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.01.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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