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Purified anti-FGF-4 Antibody

Pricing & Availability
Clone
2D1C57 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroslast Growth Factor 4 (FGF-4), Heparin-Binding Growth Factor 4 (HBGF-4), HST-1, K-FGF, KS3, Kaposi Sacrcoma Oncogene, Heparin Secretory Transforming Protein 1 (HSTF-1), FGF4, HBGF4, HST1, HSTF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2D1C57_PURE_FGF-4_Antibody_WB_063015
20 or 50 ng of human FGF-4 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-FGF-4 (clone 2D1C57) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 2D1C57_PURE_FGF-4_Antibody_WB_063015
    20 or 50 ng of human FGF-4 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-FGF-4 (clone 2D1C57) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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677402 100 µg 229 CHF
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Description

FGF-4 was first identified as HST-1 gene by a NIH3T3 transforming assay. It belongs to the FGF family that includes 22 members (FGF1-FGF23). However, FGF15 has not been identified in humans. FGF-4 exhibits strong effects on many different cell types and tissues, and plays an important role in both embryogenesis and adult tissue stem cell development in a variety of organisms. FGF-4 can stimulate limb mesenchyme proliferation and can provide all the signals required for normal outgrowth and patterning of the limb. FGF-4 mRNA has been detected in the apical ectodermal ridge of the limb bud in day 11 and 12 embryos. FGF-4 is also involved in early heart development by supporting the proliferation and differentiation of precardiac myoblasts. In adults, FGF-4 is expressed in the testis and its overexpression results in enhanced spermatogenesis. FGF-4 is also a potent inducer of platelet production from megakaryocytes. FGF-4 expression has been detected in the brain at both neonatal and adult stages. FGF-4 can induce neural stem cell proliferation and neuronal differentiation. The diverse functions of FGF-4 are mediated by FGF receptors (FGFR) that contain an extracellular ligand-binding domain with three immunoglobulin-like domains. Like other FGFs, FGF-4 also binds to anionic glycosaminoglycans heparin and heparin sulfate with a high affinity. Heparin sulfate availability has been shown to regulate the binding between FGF-4 and its receptors. The role of FGF-4 in cancer has also been extensively investigated. FGF-4 is an angiogenic protein, and the amplification of FGF-4 gene has been found in many human tumors. It has been shown that FGF-4 is a potential target for the treatment of human testicular tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human FGF-4 recombinant protein (54-206 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565907 (BioLegend Cat. No. 677402)

Antigen Details

Structure
The 153 amino acid recombinant protein has a predicted molecular mass of approximately 19.7 kD. Growth factor.
Distribution
Secreted. Expression of FGF-4 is highly restricted to undifferentiated stem cells.
Function
FGF-4 is a mitogenic, angiogenic, and survival factor. It is involved in cell proliferation and differentiation in embryonic development processes. N-glycosylation may negatively regulate FGF-4 activity.
Interaction
Precardiac myoblasts, megacariocytes, and neural stem cells.
Ligand/Receptor
FGFR-I, IIIc; FGFR-2, IIIc; FGFR-3, IIIc, and FGFR-4.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Growth Factors
Antigen References

1. Konishi H, et al. 1996. Oncogene 13:9.
2. Sun X, et al. 2000. Nat. Genet. 25:83.
3. Yamamoto H, et al. 2002. Oncogene 21:899.
4. Kosaka N, et al. 2006. FASEB J. 20:1484.
5. Kosaka N, et al. 2009. Dev. Dyn. 238:265.
6. Arao T, et al. 2013. Hepatology 57:1407.
7. Itoh N, Ohta H. 2013. Front Physiol. 4:247.

Gene ID
2249 View all products for this Gene ID
UniProt
View information about FGF-4 on UniProt.org
Go To Top Version: 1    Revision Date: 06.30.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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