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Purified anti-FMRP Antibody

Pricing & Availability
Clone
O91D10 (See other available formats)
Regulatory Status
RUO
Other Names
Fragile X Mental Retardation 1, Fragile X Mental Retardation Protein 1, Synaptic Functional Regulator FMR1, Premature Ovarian Failure 1, Protein FMR-1, FRAXA, POF1, POF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O91D10_Purified_FMRP_Antibody_040119
Western blot of purified anti-FMRP antibody (clone O91D10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • O91D10_Purified_FMRP_Antibody_040119
    Western blot of purified anti-FMRP antibody (clone O91D10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
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859001 25 µg 106 CHF
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859002 100 µg 276 CHF
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Description

Fragile X mental retardation protein 1 (FMRP) is an RNA binding protein important for normal cognitive development. In fragile X syndrome, abnormal expansion of CGG trinucleotide repeats in FMR1, the gene that encodes FMRP, leads to methylation and silencing of this gene. Altered FMPR expression levels leads to severe learning deficits or mental retardation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant protein fragment: amino acids 36-279
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801227 (BioLegend Cat. No. 859001)
AB_2801227 (BioLegend Cat. No. 859002)

Antigen Details

Structure
FMRP is a 632 amino acid protein with a molecular mass of ~71 kD.
Distribution

Tissue Distribution: Ubiquitously expressed with higher expression levels in brain tissue.
Cellular distribution: Plasma membrane, nucleus, and cytosol.

Function
FMRP has been reported to be involved in synaptic plasticity, dendritic spine maturation as well as synapse formation. FMRP may play a role in gene silencing by acting as translation regulator.
Interaction
FMRP interacts with the RNA binding proteins such as FXR1P, FXR2P and 82-FIP. FMRP can also interact with the cytoplasmic proteins CYFIP1 and CYFIP2 which then interacts with eIF4E thus linking FMRP to the RhoGTPase signaling pathway.
Ligand/Receptor
mGluR stimulation leads to increased FMRP expression.
Cell Type
Neurons
Biology Area
Neurodegeneration, Protein Synthesis
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Qiu S, et al. 2018. Mol Brain. 11(1):54
  2. Sunamura N, et al. 2018. Sci Rep. 8(1):11585
  3. Linder B, et al. 2008. Hum Mol Genet. 17(20):3236-46
  4. Gholizadeh S, et al. 2014. Neuropsychopharmacology. 39:3100.
Gene ID
2332 View all products for this Gene ID
UniProt
View information about FMRP on UniProt.org
Go To Top Version: 1    Revision Date: 04.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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