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Purified anti-FosB Antibody

Pricing & Availability
Clone
W19011B (See other available formats)
Regulatory Status
RUO
Other Names
G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W19011B_PURE_FosB_Antibody_4_030222
IHC staining of purified anti-FosB (clone W19011B) on formalin-fixed paraffin-embedded human thymus tissue. Following antigen retrieval using 1x Tris-Buffered Saline (final concentration 0.05M) w/ Tween 20 (Cat. No. 925501), the tissue was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 50 μm
  • 1_W19011B_PURE_FosB_Antibody_4_030222
    IHC staining of purified anti-FosB (clone W19011B) on formalin-fixed paraffin-embedded human thymus tissue. Following antigen retrieval using 1x Tris-Buffered Saline (final concentration 0.05M) w/ Tween 20 (Cat. No. 925501), the tissue was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 50 μm
  • 2_W19011B_PURE_FosB_Antibody_1_030222
    ICC staining of purified anti-FosB (clone W19011B) (panel A) or purified rat IgG2a, κ isotype control (Cat. No. 400501) (panel B) on HeLa cells treated with PMA (100 ng/mL) for 4 hours. The cells were fixed with 4% PFA, permeabilized with 0.5% Triton-X, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 μg/mL of the primary antibody, followed by incubation with 2.5 μg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 40X objective. Scale bar = 50 μm
  • 3_W19011B_PURE_FosB_Antibody_3_030222_updated
    Hela cells treated with 100 ng PMA for 4 hours (positive target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.50 μg of purified anti-FosB (clone W19011B) (filled histogram) or 0.50 μg of purified rat IgG2a, κ isotype control (Cat. No. 400502) (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • 4_W19011B_PURE_FosB_Antibody_2_030222_updated
    Untreated HeLa cells (negative target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.50 μg of purified anti-FosB (clone W19011B) (filled histogram) or 0.50 μg of purified rat IgG2a, κ isotype control (Cat. No. 400501) (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
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600851 25 µg 118 CHF
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600852 100 µg 293 CHF
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Description

FosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant FosB protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 5 - 10 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend fixation in 4% PFA and permeabilization for 6 minutes in 0.5% Triton-X.

Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal.

RRID
AB_2922629 (BioLegend Cat. No. 600851)
AB_2922629 (BioLegend Cat. No. 600852)

Antigen Details

Structure
FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.
Distribution

FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.

Function
FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.
Interaction
Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Tumor Suppressors
Antigen References
  1. Nestler E. 2015. Eur J Pharmacol. 753:66-72.
  2. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.
Gene ID
2354 View all products for this Gene ID
UniProt
View information about FosB on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All FosB Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-FosB W19011B IHC-P,ICC,ICFC
Alexa Fluor® 647 anti-FosB W19011B ICFC,ICC
PE anti-FosB W19011B ICFC
Go To Top Version: 1    Revision Date: 03.02.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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