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Purified anti-GALNT2 Antibody

Pricing & Availability
Clone
1501421 (See other available formats)
Regulatory Status
RUO
Other Names
Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc transferase 2, GalNAc-T2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1501421_PURE_GALNT2_Antibody_1_WB_120115
Protein (15 µg) harvested from HeLa, MCF-7, and NIH3T3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal GALNT2 (clone 1501421) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 1501421_PURE_GALNT2_Antibody_1_WB_120115
    Protein (15 µg) harvested from HeLa, MCF-7, and NIH3T3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal GALNT2 (clone 1501421) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 1501421_PURE_GALNT2_Antibody_2_IF_120115
    HeLa cells were fixed with 4% paraformaldehyde (PFA) and blocked with 5% FBS. Then the cells were stained with 2 µg/ml anti-GALNT2 antibody followed by 2 µg/mL Alexa Fluor® 594 conjugated anti-mouse IgG. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 60X objective.
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682302 100 µg 347 CHF
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Description

GALNT2 is a family member of polypeptide N-acetyl galactosaminyltransferases, which are responsible for initiating mucin type O-glycosylation by transferring GalNAc from UDP-GalNAc to Serine and Threonine residues of polypeptide acceptors. To date, there are about 20 members in this family of proteins. The characterization of peptide substrate specificity of each GALNT is critical in understanding their properties and biological roles. Trans-Golgi is the main subcellular location for GALNT2. This protein has a broad spectrum of peptide substrates such as EA2, Muc5AC, Muc1a, and Muc1b. GALNT2 may play a role in lipid metabolism. In a whole-body and liver-specific GALNT2 knockout mice study, it was shown that whole-body GALNT2 deficiency resulted in elevated VLDL-triglycerides (TG) levels as compared to wild type mice. GALNT2 KO mice did not exhibit differences in the postprandial TG clearance or VLDL-TG secretion. In mice with liver-specific deletion, reduced HDL-Cholesterol and reduced numbers of HDL particles with minimal changes in plasma TG or dietary TG clearance were observed. These findings indicate that GALNT2 may play a role as a regulator of plasma lipoprotein metabolism with tissue-specific regulatory roles.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human GALNT2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 0.1-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Goto A, et al. 2018. Traffic. 19:854. PubMed
  2. Pietrangelo A, et al. 2018. J Cell Sci. 131. PubMed
RRID
AB_2566611 (BioLegend Cat. No. 682302)

Antigen Details

Structure
519 amino acids with a predicted molecular weight of 60.3 kD.
Distribution

Golgi appartus and secreted.

Function
polypeptide N-acetyl-galactosaminyltransferases
Interaction
CCDC155 and NOD2.
Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. White T, et al. 1995. J. Biol. Chem. 41:24156-65.
2. Schjoldager KT, Clausen H. 2012. Biochim. Biophys. Acta. 12:2079.
3. Gill DJ, et al. 2011. Trends Cell Biol. 3:149.
4. Gerken TA, et al. 2011. J. Biol. Chem. 286:14493.
5. Bennett EP, et al. 2012. Glycobiology 22:736.
6. Raman J, et al. 2012. Glycobiology 22:768.

Gene ID
2590 View all products for this Gene ID
UniProt
View information about GALNT2 on UniProt.org
Go To Top Version: 1    Revision Date: 12.02.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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