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Purified anti-Gelsolin Antibody

Pricing & Availability
Clone
W18073A (See other available formats)
Regulatory Status
RUO
Other Names
GSN, Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18073A_PURE_Gelsolin_Antibody_1_072919
Whole cell extracts (15 µg protein) from the indicated cell lines, MOLT-4 and RPMI 8226 (low expressor controls), Hep G2, THP-1, HaCAT, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Gelsolin antibody, clone W18073A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
  • W18073A_PURE_Gelsolin_Antibody_1_072919
    Whole cell extracts (15 µg protein) from the indicated cell lines, MOLT-4 and RPMI 8226 (low expressor controls), Hep G2, THP-1, HaCAT, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Gelsolin antibody, clone W18073A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
  • W18073A_PURE_Gelsolin_Antibody_2_072919
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified rat IgG2a, κ Isotype Control Antibody (Cat. No. 400502) or Purified anti- Gelsolin Antibody, clone W18073A. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1.0 µg/mL (1:500 dilution) of W18073A. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
623001 25 µg 101 CHF
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623002 100 µg 253 CHF
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Description

Gelsolin (GSN) is a calcium-regulated protein that serves actin filament assembly and disassembly and regulates cytoskeletal actin organization. GSN caps the plus end of the actin-filament  to block monomer exchange, consequently, regulating filament length near the plasma membrane. Further, it contributes to cell movement and cell morphology. Four different isoforms of the molecule have been described that are produce via alternative splicing and initiation, including a soluble form. GSN is associated with a variety of diseases such as inflammation, cancer and amyloidosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human recombinant Gelsolin protein (amino acids 239-503)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814478 (BioLegend Cat. No. 623001)
AB_2814478 (BioLegend Cat. No. 623002)

Antigen Details

Structure
GSN is a 782 amino acid protein with a predicted molecular weight of 86 kD
Distribution

Actin Filaments

Function
Functions in assembly and disassembly of actin filaments.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Janmey, P.A. and Stossel, T.P., 1987. Nature. 325:362
  2. Burtnick LD, et al. 1997. Cell. 90:661.
Gene ID
2934 View all products for this Gene ID
UniProt
View information about Gelsolin on UniProt.org
Go To Top Version: 1    Revision Date: 07.29.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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