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Purified anti-Gemin 2 Antibody

Pricing & Availability
Clone
2S7 (See other available formats)
Regulatory Status
RUO
Other Names
GEMIN 2, SIP1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2S7_Purified_Gemin-2_Antibody_041019
Western blot of purified anti-Gemin 2 antibody (clone 2S7) and isotype-matched IgG1, κ control. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HeLa cell lysate. The blots were incubated with 2 µg/mL of clone 2S7 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2S7_Purified_Gemin-2_Antibody_041019
    Western blot of purified anti-Gemin 2 antibody (clone 2S7) and isotype-matched IgG1, κ control. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HeLa cell lysate. The blots were incubated with 2 µg/mL of clone 2S7 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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862901 25 µg 106 CHF
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862902 100 µg 276 CHF
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Description

Gemin 2 is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells. Gemin 2 is the only gemin protein that has no homology to any other gemins. Recent studies have suggested that the role of Gemin 2 is to organize 5 Sm proteins into a partial ring for snRNA binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Battle D, et al. 2007. J Biol Chem. 282(38):27953 (WB, ICC)
Product Citations
  1. Bizen N, et al. 2022. Cell Death Differ. . PubMed
RRID
AB_2810755 (BioLegend Cat. No. 862901)
AB_2810755 (BioLegend Cat. No. 862902)

Antigen Details

Structure
Gemin 2 is a 280 amino acid protein with an apparent molecular mass of ~ 32 kD.
Distribution

Tissue Distribution: Brain, skeletal muscle, gallbladder, and testis
Cellular Distribution: Nuclear and cytosol

Function
Gemin 2 is a component of the SMN complex that is essential for the formation of snRNP complexes. It organizes 5 Sm proteins into a partial ring for snRNA binding.
Interaction
Gemin 2 binds to SM proteins and is found in complex with other SMN/GEMIN proteins.
Cell Type
Neurons
Biology Area
Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References
  1. Battle D, et al. 2007. J Biol Chem. 282(38):27953
  2. Gupta K, et al. 2015. J Biol Chem. 290(33):20185
Gene ID
8487 View all products for this Gene ID 66603 View all products for this Gene ID 84404 View all products for this Gene ID
UniProt
View information about Gemin 2 on UniProt.org
Go To Top Version: 1    Revision Date: 04.10.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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