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Purified anti-GGT1 Antibody

Pricing & Availability
Clone
BL28543 (See other available formats)
Regulatory Status
RUO
Other Names
GGT1, Gamma-glutamyltransferase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL28543_Pure_GGT1_Antibody_1_111218
Western blot of purified anti-GGT1 antibody (clone BL28543). Lane 1: Molecular weight marker; Lane 2: 20 µg of HepG2 Cell lysate; Lane 3: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28543_Pure_GGT1_Antibody_1_111218
    Western blot of purified anti-GGT1 antibody (clone BL28543). Lane 1: Molecular weight marker; Lane 2: 20 µg of HepG2 Cell lysate; Lane 3: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28543_Pure_GGT1_Antibody_2_111218
    IHC staining of purified anti-GGT1 antibody (clone BL28543) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Check Availability Save
854301 25 µg 106 CHF
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Description

GGT1 is a transferase that plays a key role in the gamma-glutamyl cycle. GGT1 is abundantly expressed in liver and kidney. Its involvement in glutathione metabolism makes it an important regulator of the oxidative stress in the central nervous system, as well as in other tissues. GGT1 is also a known marker for apoptosis. Importantly, elevated levels of GGT1 correlates with breast cancer tumor progression and resistance to chemotherapy, suggesting GGT1 as a candidate for cancer prognosis marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783438 (BioLegend Cat. No. 854301)

Antigen Details

Structure
GGT1 is a 569 amino acid protein with a molecular mass of ~ 61 kD.
Distribution

Tissue Distribution: Kidney, liver, pancreas, stomach, intestine, placenta, and lung.
Cellular distribution: Mainly on plasma membrane or extracellular

Function
Cleaves the gamma-glutamyl bond of extracellular glutathione, maintains intracellular GSH level.
Interaction
Heterodimer forms by interactions between light and heavy chains.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Priolo C, et al. 2018. Proc Natl Acad Sci U S A. 115(27):E6274
  2. Darin N, et al. 2018. Eur J Hum Genet. 26(6):808
  3. Yoshikawa C. 1995. Nihon Rinsho. 53(5): 1162
Gene ID
2678 View all products for this Gene ID
UniProt
View information about GGT1 on UniProt.org
Go To Top Version: 1    Revision Date: 11.14.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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