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Purified anti-Glutamine synthetase Antibody

Pricing & Availability
Clone
O91F4 (See other available formats)
Regulatory Status
RUO
Other Names
GLUL, Glutamine Synthetase
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
O91F4_Purified_Glutamine-Synthetase_Antibody_1_100918
Western blot of purified anti-Glutamine synthetase antibody (clone O91F4). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O91F4_Purified_Glutamine-Synthetase_Antibody_1_100918
    Western blot of purified anti-Glutamine synthetase antibody (clone O91F4). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O91F4_Purified_Glutamine-Synthetase_Antibody_2_100918
    IHC staining of purified anti-Glutamine synthetase antibody (clone O91F4) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • O91F4_Purified_Glutamine-Synthetase_Antibody_4_100918
    IHC staining of purified anti-Glutamine synthetase antibody (clone O91F4) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • O91F4_Purified_Glutamine-Synthetase_Antibody_5_022520
    IHC staining of purified anti-Glutamine synthetase antibody (clone O91F4) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The slide was mounted with fluoromount G with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • O91F4_Purified_Glutamine-Synthetase_Antibody_6_022520
    IHC staining of purified anti-Glutamine synthetase antibody (clone O91F4) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The slide was mounted with fluoromount G with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • O91F4_Purified_Glutamine-Synthetase_Antibody_7_022520
    ICC staining of purified anti-Glutamine synthetase antibody (clone O91F4) on SH-SY5Y cell. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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856201 25 µg 106 CHF
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856202 100 µg 276 CHF
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Description

Glutamine Synthase (GLUL) is primarily expressed in astrocytes in the brain. The main function of GLUL is to catalyze the condensation of glutamate and ammonia to form glutamine. GLUL plays an important role in the metabolic regulation of glutamate, detoxification of brain ammonia, as well as recycling of neurotransmitters. GLUL expression in endothelial cells may be involved in cell migration during pathological angiogenesis. Upregulation of astrocytic GLUL to uptake excess ammonia and glutamate may play a neuroprotective role during neuroinflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human GLUL protein expressed in HEK293T cell
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL for chromogenic staining and 0.5 - 5.0 µg/mL for fluorescent staining is suggested. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Shajahan-Haq AN, et al. 2014. Mol Cancer. 13:239. (WB)
RRID
AB_2783452 (BioLegend Cat. No. 856201)
AB_2783452 (BioLegend Cat. No. 856202)

Antigen Details

Structure
GLUL is a 373 amino acid protein with an apparent molecular mass of ~ 42 kD.
Distribution

Tissue Distribution: Predominantly expressed in brain, kidney, and liver.
Cellular Distribution: Extracellular, cytosol, nucleus, and mitochondrion.

Interaction
Forms homooctamer and homotetramer.
Cell Type
Astrocytes
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Eelen G, et al. 2018. Nature. (7721):63-69
  2. Spodenkiewicz M, et al. 2016. Biology (Basel). 5(4)
  3. Fan S, et al. 2018. J Cell Biochem. 119(7):6008
Gene ID
2752 View all products for this Gene ID 14645 View all products for this Gene ID 24957 View all products for this Gene ID
UniProt
View information about Glutamine synthetase on UniProt.org
Go To Top Version: 2    Revision Date: 02.25.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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