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Purified anti-Glycodelin Antibody

Pricing & Availability
Clone
A16006H (See other available formats)
Regulatory Status
RUO
Other Names
Placental protein 14, PP14, Progestagen-associated endometrial protein, Progesterone-associated endometrial protein, PAEP, Zona-binding inhibitory factor-1. ZIF-1, Pregnancy-associated endometrial alpha-2 globulin, PAEG, PEG
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16006H_PURE_Glycodelin_WB_062223
Whole cell extracts (15 µg total protein) from the indicated tissues were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-Glycodelin (clone A16006H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A16006H_PURE_Glycodelin_WB_062223
    Whole cell extracts (15 µg total protein) from the indicated tissues were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-Glycodelin (clone A16006H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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616351 25 µg 132 CHF
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616352 100 µg 330 CHF
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Description

Glycodelin, also known as progesterone-associated endometrial protein (PAEP), belongs to the lipocalin family and exists in four glycoforms. The glycodelin isoforms are primarily expressed in different reproductive tissues, such as glycodelin-S in male seminal vesicles, glycodelin-A (GdA) in the endometrium, glycodelin-F (GdF) in fallopian tubes/follicular fluid, and glycodelin-C (GdC) in cumulus cells. Glycodelin isoforms are involved in reproductive processes such as implantation, fertilization, and pregnancy maintenance. GdA plays a crucial role in differentiating natural killer (NK) cells to decidual NK cells and modulating the NK cells to prevent maternal immune rejection during embryo development. At the feto-maternal interface, GdA inhibits the proliferation of T-cells and induces their apoptosis through Th receptors. GdC is converted from GdA and GdF through glycosylation modification and facilitates sperm-zona pellucida binding. Recently, abnormal glycodelin expression has been observed in various cancers, including ovarian, breast, colon, and lung cancers. Mice xenograft models have revealed that glycodelin plays a role in tumor proliferation and invasion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human glycodelin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in immunohistochemistry.

RRID
AB_2941624 (BioLegend Cat. No. 616351)
AB_2941624 (BioLegend Cat. No. 616352)

Antigen Details

Structure
Forms four glycoforms, glycodelin-S, -A, -F and -C; homodimer
Distribution

Expressed in and secreted by reproductive tissues

Function
Participates in reproductive processes, including implantation, fertilization, and pregnancy maintenance
Interaction
A2M, SIGLEC6
Biology Area
Immunology
Antigen References
  1. Seppala M, et al. 2007. Hum Reprod Update. 3:275-87.
  2. Ren S, et al. 2010. J Cell Mol Med. 4:1432-42.
  3. Lee C-L, et al. 2011. J Repord Immunol. 90:29-34.
  4. Cui J, et al. 2017. Front Immunol. 8:1685.
  5. Lee C-L, et al. 2019. Hum Reprod. 34:689-701.
Gene ID
5047 View all products for this Gene ID
UniProt
View information about Glycodelin on UniProt.org
Go To Top Version: 1    Revision Date: 06.22.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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