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Purified anti-Granzyme B Antibody

Pricing & Availability
Clone
O94E6/GZMB (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, CSPB, CTLA-1, CGL-1, CGL1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
O94E6-GZMB_Purified_Granzyme B_Antibody_1WB_112514
Western blot analysis of 50ng of recombinant human Granzyme B (lane 1), Daudi (lane 2), Jurkat (lane 3), and PBMC (lane 4) using anti-Granzyme B antibody (clone O94E6/GZMB). Anti-GAPDH antibody was used as a loading control.
  • O94E6-GZMB_Purified_Granzyme B_Antibody_1WB_112514
    Western blot analysis of 50ng of recombinant human Granzyme B (lane 1), Daudi (lane 2), Jurkat (lane 3), and PBMC (lane 4) using anti-Granzyme B antibody (clone O94E6/GZMB). Anti-GAPDH antibody was used as a loading control.
  • O94E6-GZMB_Purified_Granzyme B_Antibody_2WB_112514
    Western blot analysis of PBMC (lane 1), PBMC activated with CD3 and CD28 antibody for two days (lane 2), and mouse thymus (lane 3) using anti-Granzyme B antibody(clone O94E6/GZMB ). Anti-β actin antibody was used as a loading control.
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674301 25 µg 101 CHF
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674302 100 µg 300 CHF
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Description

Granzyme B is a serine protease that is produced mainly by cytotoxic T cells (CTL) and natural killer (NK) cells. It plays an important role in cytotoxic lymphocyte-mediated apoptosis, chronic inflammation, and impaired wound healing. Granzyme B is packaged in cytoplasmic granules that are exocytosed towards a bound target cell, which subsequently activates multiple protein substrates that induce apoptosis. Perforin facilitates the transfer of Granzyme B into the target cell. Most circulating CD56+, CD8-, NK cells, and approximately half of circulating CD8+ T cells coexpress both Granzyme A and B. The activation of CD8+ and CD4+ T lymphocytes induce substantial expression of Granzyme B, but not Granzyme A. Recently, Granzyme B has been found to be produced by other cell types such as CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, B cells, and smooth muscle cells. Elevated levels of Granzyme B are also found in some kinds of autoimmune diseases, type 1 diabetes, and cardiovascular diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment corresponding to amino acids 21-247 of Human Granzyme B produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at 4°C
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Azab W, et al. 2012. J Virol. 2031:86. PubMed
  2. Mo F, et al. 2021. Nature. 597:544. PubMed
RRID
AB_2564582 (BioLegend Cat. No. 674301)
AB_2564582 (BioLegend Cat. No. 674302)

Antigen Details

Structure
247 amino acids with a molecular weight of approximately 28 kD.
Distribution

Cytotoxic T cells, Natural killer (NK) cells, and neutrophils.

Function
Induces target cell apoptosis by activating caspase independent pathways and is induced in CD8+ T lymphocytes with ConA / IL-2 and CD4+ lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Targets of CTL and NK cells.
Cell Type
B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 1:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6934.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 12:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.
8. Hiebert PR, et al. 2012. Trends Mol. Med. 18:732.
9. Ewen CL, et al. 2012. Cell Death Differ. 1:28.

Gene ID
3002 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org
Go To Top Version: 2    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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