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Purified anti-HAGH Antibody

Pricing & Availability
Clone
W22099D (See other available formats)
Regulatory Status
RUO
Other Names
HAGH1, Hydroxyacylglutathione Hydrolase, Hydroxyacylglutathione Hydrolase, Mitochondrial, Glyoxalase II, GLO2, GLX2, GLO-2, Q16775
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W22099D_PURE_HAGH_WB_082024
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-HAGH (clone W22099D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W22099D_PURE_HAGH_WB_082024
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-HAGH (clone W22099D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W22099D_PURE_HAGH_IHC-P_082024
    IHC staining of Purified anti-HAGH (clone W22099D) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with (panels A and B) and without (panels C and D) Purified anti-HAGH (clone W22099D) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panels A and C) or 40X (panels B and D) objective and merged. Scale bar: 50 µm
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682951 25 µg 180 CHF
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682952 100 µg 445 CHF
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Description

Hydroxyacylglutathione hydrolase, mitochondrial or GLO2, is integral to cellular detoxification and metabolic regulation. It operates within the glyoxalase system, a critical pathway for neutralizing methylglyoxal, a highly reactive and cytotoxic byproduct of glycolysis. The glyoxalase system includes two primary enzymes: Lactoylglutathione lyase (GLO1) and HAGH/GLO2. GLO1 catalyzes the reaction between methylglyoxal and glutathione (GSH) to form S-D-lactoylglutathione. Subsequently, HAGH/GLO2 hydrolyzes S-D-lactoylglutathione to form D-lactate and regenerates GSH. This detoxification is crucial as methylglyoxal can cause significant cellular damage by glycation, which impairs the function of proteins, nucleic acids, and lipids. By mitigating this damage, HAGH helps protect cellular components and maintain cellular integrity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human HAGH (GLO2)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W22099D) is not suitable for immunocytochemistry (ICC).

For immunohistochemistry on formalin-fixed parafilm-embedded tissue (IHC-P), the recommended antigen retrieval is either Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704) or Citrate Buffer (Cat. No.420902).

RRID
AB_3662337 (BioLegend Cat. No. 682951)
AB_3662337 (BioLegend Cat. No. 682952)

Antigen Details

Structure
HAGH/GLO2 is a 308 amino acid protein with a predicted molecular weight of 34 kD.
Distribution

Highly expressed in liver and kidney. Localizes in the cytosol and/or mitochondrial matrix.

Function
Catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References
  1. Rabbani N, et al. 2014. Biochem Soc Trans. 42:419-24.
  2. Bangel FN, et al. 2015. Prog Neuropsychopharmacol Biol Psychiatry. 59:105-110.
  3. Gaffney DO, et al. 2020. Cell Chem Biol. 27:206-213.
Gene ID
3029 View all products for this Gene ID
UniProt
View information about HAGH on UniProt.org
Go To Top Version: 1    Revision Date: 08.20.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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