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Purified anti-HDAC2 Antibody

Pricing & Availability
Clone
13G8C67 (See other available formats)
Regulatory Status
RUO
Other Names
Histone Deacetylase 2, YY1-Associated Factor 1, HD2, Transcriptional Regulator Homolog RPD3, YAF1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_13G8C67_PURE_HDAC2_Antibody_1_WB_092915_updated
Total lysates (15 µg protein) from 293T (lane 1) and 293T/HDAC2 knockdown (KD) cells (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-HDAC2 Antibody, clone 13G8C67 (upper) or 1:3000 diluted Purified anti-GAPDH Antibody, clone poly6314 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC2 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 1_13G8C67_PURE_HDAC2_Antibody_1_WB_092915_updated
    Total lysates (15 µg protein) from 293T (lane 1) and 293T/HDAC2 knockdown (KD) cells (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-HDAC2 Antibody, clone 13G8C67 (upper) or 1:3000 diluted Purified anti-GAPDH Antibody, clone poly6314 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC2 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 2_13G8C67_PURE_HDAC2_Antibody_2_WB_092915
    Immunoprecipitation of HDAC2 from 293T cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-HDAC2 antibody (clone 13G8C67, lane 4). Western blot was performed using anti-HDAC2 antibody (clone 13G8C67).
  • 3_13G8C67_PURE_HDAC2_Antibody_3_ICC_042319
    HepG2 cells were stained with purified anti-HDAC2 (clone 13G8C67) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibodies, and then Alexa Fluor® 594 conjugated phalloidin (red).
  • 4_13G8C67_GoChIP_HDAC2_Antibody_1_ChIP_120916.jpg
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC2 Antibody (clone 13G8C67), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (clone MG1-45), or C) competitor's ChIP-grade Purified anti-HDAC2 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human FOSL1 (Fos like 1) gene region, which is known to be bound to HDAC2. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
Cat # Size Price Quantity Check Availability Save
680101 25 µg 112 CHF
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680102 100 µg 276 CHF
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Description

HDAC2 is a member of the class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups from the side chain of lysine residues on the N-terminal region of core histones. HDAC2 lacks DNA binding activity and executes its function by recruiting transcription factors and forming large transcriptional repressor complexes. In addition to histones, HDAC2 can deacetylate transcription factors and modify their transcriptional activity. HDAC2 is highly homologous to HDAC1 in regulating cell proliferation, differentiation, and apoptosis. HDAC2 has been shown to negatively regulate synaptic plasticity and memory formation. Elevated activity of HDAC2 has been associated with the loss of memory and neuronal degeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human HDAC2 peptide (471-488 a.a.) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, KO/KD-WB, ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10 - 30 μg per ml. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A minor non-specific band of ~70 kDa was observed by western blot during product development.

RRID
AB_2566332 (BioLegend Cat. No. 680101)
AB_2566332 (BioLegend Cat. No. 680102)

Antigen Details

Structure
488 amino acids with a predicted molecular weight of 55.4 kD. It contains a histone deacetylase domain.
Distribution

Nucleus.

Function
HDAC2 is a histone deacetylase responsible for the deacetylation of ε-amino acid group of lysine residues on the N-terminal tail of core histones. Forms of complex with transcription factors and acts as a transcriptional co-repressor.
Interaction
Forms histone deacetylase complexes with HDAC1, RBBP4, and RBBP7. Interacts with GFI1, SNW1, HDAC7, PRDM6, SAP30, SETDB1, SUV39H1, H2AFY, ATR, CBFA2T3, BCL-6, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A, and PELP1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References

1. Winter M, et al. 2013. EMBO J. 32:3176.
2. Ma P, et al. 2013. PLoS Genet. 9:e1003377.
3. Kurita M, et al. 2012. Nat. Neurosci. 15:1245.
4. Segre CV, et al. 2011. J. Biomed. Biotechnol. 2011:690848.
5. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275.
6. Guan JS, et al. 2009. Nature 459:55.

Gene ID
3066 View all products for this Gene ID
UniProt
View information about HDAC2 on UniProt.org

Other Formats

View All HDAC2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-HDAC2 13G8C67 WB,IP,ICC,KO/KD-WB,ChIP
Direct-Blot™ HRP anti-HDAC2 13G8C67 WB
Go To Top Version: 5    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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