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Purified anti-HDAC8 Antibody

Pricing & Availability
Clone
W15090A (See other available formats)
Regulatory Status
RUO
Other Names
Histone deacetylase-like 1 (HDACL1), MRXS6, Wilson-Turner X-linked mental retardation syndrome (WTS), RPD3
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_W15090A_Pure_HDAC8_Antibody_WB_110117
Total lysates (10µg protein) from HeLa and HeLa HDAC8 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-HDAC8 antibody , clone W15090A (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-HDAC8 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder.
  • 1_W15090A_Pure_HDAC8_Antibody_WB_110117
    Total lysates (10µg protein) from HeLa and HeLa HDAC8 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-HDAC8 antibody , clone W15090A (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-HDAC8 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder.
  • 2_W15090A_Pure_HDAC8_Antibody_1_WB_033016
    Total lysates (15 µg protein) mouse brain (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-HDAC8 Antibody, clone W15090A (upper) or 1:3000 diluted Purified anti-GAPDH Antibody, clone poly6314 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC8 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody.
  • 3_W15090A_Pure_HDAC8_Antibody_2_ICC_041519
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2 µg/ml purified anti-human HDAC8 (clone W15090A) antibody for one hour, followed by staining with Alexa Fluor® 594 anti-mouse IgG antibody (red), Alexa Fluor® 488 Phalloidin (green), and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
  • 4_W15090A_GoChIP_HDAC8_Antibody_042617
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC8 Antibody (Clone W15090A), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-HDAC8 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR-1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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685502 100 µg 311 CHF
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Description

Histone deacetylases (HDACs) are a family of enzymes responsible for the removal of acetyl groups from lysine residues on the core histones and other non-histone proteins. HDAC8 is a type I histone deacetylase and was initially found to deacetylate a variety of acetylated histone variants in vitro. HDAC8 is a kinectically and structurally well-characterized deacetylase that was reported to catalyze several nuclear proteins, which include ERR-α, hEST1B, and SMC3. Patients with HDAC8 mutations show Cornelia de Lange Syndrome (CdLS) and Wilson-turner Syndrome (WTS) phenotypes. Elevated expression of HDAC8 has been observed in various tumor types, such as acute lymphoblastic leukemia (AML), neuroblastomas, and glimoas. HDAC8 is implicated as a therapeutic target in cancer and infectious diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human HDAC8 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC, ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Aru B, et al. 2020. ACS Omega. 5:25854. PubMed
RRID
AB_2572204 (BioLegend Cat. No. 685502)

Antigen Details

Structure
377 amino acids with a predicted molecular weight of 41.8 kD. It belongs to the type I Histone deacetylase subfamily.
Distribution

Nucleus and cytoplasm.

Function
HDAC8 is a deacetylase and regulates gene expression through catalyzing the removal of acetyl groups on lysine residues in the core histones and transcriptional factors.
Interaction
Interacts with PEPB2-MYH11, CBFA2T3, SMG5, and alpha-SMA.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Qi J, et al. 2015. Cell Stem Cell. 17:597.
2. Tian Y, et al. 2015. Cancer Res. 75:4803.
3. Feng L, et al. 2015. J. Hum. Genet. 60:165.
4. Ha SD, et al. 2014. J. Immunol. 193:1333.
5. Higuchi T, et al. 2013. Blood 121:3640.
6. Deardorff MA, et al. 2012. Nature 489:313.

Gene ID
55869 View all products for this Gene ID
UniProt
View information about HDAC8 on UniProt.org

Other Formats

View All HDAC8 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-HDAC8 W15090A WB,KO/KD-WB,ICC,ChIP
Go To Top Version: 4    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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