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Purified anti-HIF1-β Antibody

Pricing & Availability
Clone
W15091A (See other available formats)
Regulatory Status
RUO
Other Names
Aryl hydrocarbon receptor nuclear translocator (ARNT), Class E basic helix-loop-helix protein 2 (bHLHe2), Hypoxia-inducible factor 1-beta
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15091A_PURE_HIF1-beta_Antibody_1_102218
Total cell lysates (15 µg total protein) from THP-1, SH-SY5Y, MCF-7, HeLa, Molt-4, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-HIF1-β Antibody, clone W15091A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W15091A_PURE_HIF1-beta_Antibody_1_102218
    Total cell lysates (15 µg total protein) from THP-1, SH-SY5Y, MCF-7, HeLa, Molt-4, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-HIF1-β Antibody, clone W15091A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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689602 100 µg 253 CHF
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Description

Hypoxia-inducible factor 1 beta (HIF1-β), also known as Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), functions mainly as a transcription factor. This protein contains a basic helix-loop-helix domain, two PAS (PER-ARNT-SIM) domains and one PAC (PAS-associated c-terminal) domain. HIF1-β binds to the ligand-bound aryl hydrocarbon (Ah) receptor and helps the complex move from cytosol to the nucleus, where it promotes the expression of xenobiotic metabolism-related genes. HIF1-β is also involved in cellular senescence and HIF-1 transcription factor network. It forms a heterodimer with HIF1-α or EPAS1/HIF2A and transcriptionally regulates the response to hypoxia. There are multiple transcript variants of HIF1-β generated by alternative splicing.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human HIF1-β recombinant protein (517-691 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W15091A does not cross-react with mouse.

RRID
AB_2629788 (BioLegend Cat. No. 689602)

Antigen Details

Structure
789 amino acids with a predicted molecular weight of 86.6 kD. Contains a bHLH (basic helix-loop-helix) domain, a PAC (PAS-associated C-terminal) domain, and two PAS (PER-ARNT-SIM) domains.
Distribution

Nucleus

Function
HIF1-β interacts with ligand-bound aryl hydrocarbon receptor (AhR) and acts as a transcriptional co-activator for the genes involved in xenobiotic metabolism.
Interaction
Forms a heterodimer with AHR, AHRR, HIF1A, EPAS1/HIF2, and other bHLH proteins. Interacts with TACC3 and NOCA7.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Furue M, et al. 2014. Arch Dermatol. Res. 306:769.
2. Vorrink SU, et al. 2014. Chem. Biol. Interact. 218:82.
3. Mandl M, et al. 2014. Mol. Med. 20:215.
4. Gu C, et al. 2014. Leuk. Res. 37:1750.
5. Wolff M, et al. 2013. Cell Physiol Biochem. 32:849.
6. Nakajima K, et al. 2013. Nat. Commun. 4:2112.

Gene ID
405 View all products for this Gene ID
UniProt
View information about HIF1-beta on UniProt.org
Go To Top Version: 2    Revision Date: 10.22.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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