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Purified anti-Histone H3 Acetylated Lysine 9 (K9ac) Antibody

Pricing & Availability
Clone
2G1F9 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
2G1F9_PURE_Histone_H3_Acetylated-Lysine9_Antibody_071817
Total cell lysates (15µg protein) from NIH/3T3 and HeLa cells treated with (+) or without (-) 5 mM Na-Butyrate for 20 hours were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-K9ac H3 (clone 2G1F9, left) antibody and a competitor’s antibody used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using goat anti-rat-IgG (for 2G1F9, cat #405405) or donkey anti-rabbit-IgG secondary antibodies conjugated to HRP (for competitor’s clone, cat #406401). Total Histone H3 (cat# 601902) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • 2G1F9_PURE_Histone_H3_Acetylated-Lysine9_Antibody_071817
    Total cell lysates (15µg protein) from NIH/3T3 and HeLa cells treated with (+) or without (-) 5 mM Na-Butyrate for 20 hours were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-K9ac H3 (clone 2G1F9, left) antibody and a competitor’s antibody used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using goat anti-rat-IgG (for 2G1F9, cat #405405) or donkey anti-rabbit-IgG secondary antibodies conjugated to HRP (for competitor’s clone, cat #406401). Total Histone H3 (cat# 601902) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • 2G1F9_PURE_Histone_H3_Acetylated-Lysine9_Antibody_2_072519
    Untreated (panel A) and sodium butyrate-treated (5 mM for 16 hours, panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells treated were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of Purified anti-Histone H3 Acetylated Lysine 9 Antibody, clone 2G1F9, overnight at 4°C, after which proteins were visualized with Alexa Fluor® 488 Goat anti-mouse IgG Antibody (Cat. No. 405319) at 1.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
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698401 25 µg 101 CHF
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698402 100 µg 253 CHF
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Description

Histone subunit H3, along with subunits 2A, 2B, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Histone H3 K9ac peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 1.0 µg per ml (1:500-1:50000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2715783 (BioLegend Cat. No. 698401)
AB_2715783 (BioLegend Cat. No. 698402)

Antigen Details

Structure
Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational.
Distribution

Nucleus

Function
Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones.
Interaction
Histones 2A, 2B, 4, HDAC
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Zhang, et al. 2017. Nat. Commun. 8: 14799.
2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394.
3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333.
4. Li B, et al. 2016. Sci. Rep. 6: 26542.
5. Li D, et al. 2014, Mol. Cancer. 13:26.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 2    Revision Date: 07.19.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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