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Purified anti-HOMER1 Antibody

Pricing & Availability
Clone
A18192B (See other available formats)
Regulatory Status
RUO
Other Names
Homer protein homolog 1 (HOMER1), SYN47
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18192B_PURE_HOMER1_Antibody_WB_020723
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-HOMER1 (clone A18192B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • A18192B_PURE_HOMER1_Antibody_WB_020723
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-HOMER1 (clone A18192B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
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610551 25 µg 120 CHF
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610552 100 µg 375 CHF
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Description

HOMER1 is a scaffolding protein that is a major component of the post-synaptic density (PSD), a region in the plasma membrane of the post-synaptic neuron that interacts with the pre-synaptic active zone of upstream neurons. HOMER1 consists of two domains, a C-terminal coiled-coil domain that is required for HOMER1 dimerization, and an EVH1 domain involved in protein-protein interaction. Dimerized long-form HOMER1 (variants designated as Homer1b and Homer1c) is constitutively expressed in neurons, where it plays a role in neurotransmitter receptor signaling and trafficking by cross-linking associated proteins via the EVH1 domain. Truncated HOMER1 (Homer1a) is expressed as a result of neuronal activity, and lacking the coiled-coil domain, is incapable of dimerizing and cannot crosslink interacting proteins. Thus, Homer1a acts as a dominant-negative regulator of HOMER1 and is thought to provide negative feedback against excessive neuronal activity. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human HOMER1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in Immunohistochemistry.

This product does not recognize mouse or human HOMER1 in Western Blot.

This product only recognizes full length HOMER1 (Homer1b/c) and not the truncated variant Homer1a.

RRID
AB_2936737 (BioLegend Cat. No. 610551)
AB_2936737 (BioLegend Cat. No. 610552)

Antigen Details

Structure
HOMER1 is a 354 amino acid protein with a predicted molecular weight of 40 kD.
Distribution

Post-synaptic density

Function
Protein-protein interactions, synaptic plasticity
Interaction
Homodimer; also interacts with other major components of the PSD including Shank family proteins, cytoskeletal proteins like dynamin, and receptors such as group 1 metabotropic glutamate receptors and the ryanodine receptor.
Cell Type
Mature Neurons, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins, Scaffold Proteins
Antigen References
  1. Foa L, et al. 2001. Nat Neurosci. 4:499-506. 
  2. de Bartolomeis A, et al. 2022. Neurosci Biobehav Rev. 136:104596. 
  3. Salanova M, et al. 2013. IUBMB Life. 65:769-76. 
  4. Reshetnikov VV, et al. 2021. Biochemistry (Mosc). 86:613-626. 
Gene ID
9456 View all products for this Gene ID
UniProt
View information about HOMER1 on UniProt.org
Go To Top Version: 1    Revision Date: 02.07.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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