- Clone
- W22219B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Homeobox protein Hox-B4, Homeobox protein Hox-2.6, Homeobox protein Hox-2F, Homeobox B4, HOX2F
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
661451 | 25 µg | 170 CHF | ||||
661452 | 100 µg | 430 CHF |
HOXB4 is a member of the Homeobox (HOX) gene family, which plays a role in regulating embryonic development and maintaining adult tissue homeostasis. HOXB4 is a sequence-specific transcription factor with a homeobox DNA-binding domain. In hematopoiesis, HOXB4 enhances the self-renewal capacity of hematopoietic stem cells (HSCs), which are responsible for the continuous production of blood cells. Overexpression of HOXB4 in HSCs has been shown to lead to an expansion of these stem cells without inducing leukemogenesis. By binding to specific DNA sequences, HOXB4 activates a network of target genes that promote stem cell proliferation and inhibit differentiation, thus maintaining the stem cell pool.
HOXB4 also plays a pivotal role in organogenesis, particularly in the development of the spinal cord and the patterning of the vertebral column. During embryonic development, HOXB4 is expressed in a specific spatial and temporal manner, contributing to the formation of structures along the anterior-posterior axis. Its expression helps determine the identity and positional information of various segments, ensuring that body parts develop in the correct locations. In vertebrates, this function is crucial for the proper segmentation and alignment of the spine, as well as for the formation of the nervous system. Furthermore, aberrations in HOXB4 expression or function have been associated with various pathological conditions, including cancers. Dysregulated HOXB4 expression can lead to the uncontrolled proliferation of cells, contributing to tumorigenesis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human HOXB4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is not suitable for use in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P) or immunocytochemistry (ICC).
This antibody does not cross react with mouse HOXB4 in Western blotting (WB).
Antigen Details
- Structure
- Contains a homeobox DNA-binding domain
- Distribution
-
Expressed in embryonic tissue and hematopoietic stem cells/localized in nucleus
- Function
- A transcription factor that regulates hematopoietic stem cell self-renewal or development of anterior-posterior axis
- Interaction
- Binds to homeobox DNA sequence
- Biology Area
- Neuroscience, Stem Cells, Transcription Factors
- Antigen References
-
- Gould A, et al. 1998. Neuron. 21:39-51.
- Antonchuk J, et al. 2002. Cell. 109:39-45.
- Kyba M, et al. 2002. Cell. 109:29-37.
- Bowles KM, et al. 2006. Stem Cells. 24:1359-69.
- Gene ID
- 3214 View all products for this Gene ID
- UniProt
- View information about HOXB4 on UniProt.org
Related FAQs
Other Formats
View All HOXB4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HOXB4 Antibody | W22219B | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-HOXB4 Antibody
Whole cell extracts (15 µg total protein) from indicated cel...
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