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Purified anti-HSC70 (HSPA8) Antibody

Pricing & Availability
Clone
9/2 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Shock 70 kD Protein 8, HSP70, HSP73, HSPA10, Heat Shock Cognate Protein 54, HSC54, heat stress cognate 70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
9slash2_PURE_HSC70_HSPA8_Antibody_WB_122315
Total lysates (15 µg protein) from 293T and 293T/HSC70 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-HSC70 (HSPA8) antibody, clone 9/2 (upper) or 1:3000 diluted anti-GAPDH (poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) for anti-HSC70 (HSPA8) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat. No. 406401) for GAPDH. Lane M: MW ladder.
  • 9slash2_PURE_HSC70_HSPA8_Antibody_WB_122315
    Total lysates (15 µg protein) from 293T and 293T/HSC70 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-HSC70 (HSPA8) antibody, clone 9/2 (upper) or 1:3000 diluted anti-GAPDH (poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) for anti-HSC70 (HSPA8) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat. No. 406401) for GAPDH. Lane M: MW ladder.
  • 9slash2_PURE_HSC70_HSPA8_Antibody_WB_2_122315
    Western blot analysis of cell lysates from HeLa (lane 1), THP-1 (lane 2), HEK293 (lane 3), and Raw264.7 (lane 4). Anti-HSC70 antibody (clone 9/2) was used and followed by HRP anti-mouse IgG antibody. GAPDH antibody (clone poly6314) was used as a loading control.
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682102 100 µg 265 CHF
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Description

Heat shock cognate 71 kD protein or HSC70 (HSPA8) is one of the major constitutively expressed, cognate protein chaperones to supply a multitude of the housekeeping chaperoning functions. As a chaperone, HSC70 binds to nascent polypeptides to facilitate correct folding. Besides the chaperone functions, secreted HSC70 is involved in inflammatory signal pathways via extracellular interaction with TLR2/TLR4. It also plays a central role in modulating antigen transport within cells to control MHC class II presentation during nutrient stress. Furthermore, HSC70 may serve as a molecular switch to modulate endocytic and autophagy pathways.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human HSC70 fragment (1-1755bp of Acc. Y00371)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566574 (BioLegend Cat. No. 682102)

Antigen Details

Structure
646 amino acids with a predicted molecular weight of approximately 71 kD.
Distribution

Cytoplasm, nucleus, and cell membrane.

Function
Chaperone and antigen transport.
Interaction
PACRG, HSPH1/HSP105, IRAK1BP1, BAG1, DNAJC7, DNAJB14, E3 ligase CHIP, CITED1 , CDC5L, PLRG1 and BCAS2, SV40 VP1, TRIM5, METTL21A, PARK2, and FOXP3.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors
Molecular Family
Heat Shock Proteins, Protein Kinases/Phosphatase
Antigen References

1. Deffit SN, Blum JS. 2015. Mol. Immunol. 68:85-8.
2. Pratt WB, et al. 2015. Annu. Rev. Pharmacol. Toxicol. 55:353.
3. Stricher F, et al. 2013. Autophagy 9:1937.
4. Liao Y, et al. 2014. Curr. Pharm. Des. 20:101.
5. Höhfeld J. 1998. Biol. Chem. 379:269.

Gene ID
3312 View all products for this Gene ID
UniProt
View information about HSC70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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