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Purified anti-HSF1 Antibody (Previously Covance catalog# MRT-5113)

Pricing & Availability
Clone
4B4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Heat shock factor protein 1, heat shock transcription factor 1, HSF 1, HSTF 1
Previously
Covance Catalog# MRT-5113
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
4B4_Purified_HSF1_Antibody_IHC_021715
IHC staining of purified anti-HSF1 antibody (clone 4B4) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Following a biotinylated goat anti-rat linking reagent, BioLegend’s Ultra-Streptavidin (USA) HRP labeling reagent and DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective
  • 4B4_Purified_HSF1_Antibody_IHC_021715
    IHC staining of purified anti-HSF1 antibody (clone 4B4) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Following a biotinylated goat anti-rat linking reagent, BioLegend’s Ultra-Streptavidin (USA) HRP labeling reagent and DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective
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825801 100 µL 275 CHF
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Description

HSF1 is a DNA-binding protein that exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90.  Upon stress, HSF1 is released from the chaperone complex and trimerizes.  HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements (HSE) and activates transcription of proteins such as HSP70.  HSP70 is known to inhibit the tau and alpha synuclein aggregation seen in Alzheimer’s Disease and Parkinson’s Disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against mouse recombinant HSF-1 protein and was mapped to amino acids 425-439.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Mathew A, et al. 2001. Mol. Cell. Biol. 21:7163. (WB, ICC)
RRID
AB_2564892 (BioLegend Cat. No. 825801)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
3297 View all products for this Gene ID
UniProt
View information about HSF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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