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Purified anti-HSPB6 Antibody

Pricing & Availability
Clone
BL28582 (See other available formats)
Regulatory Status
RUO
Other Names
Heat shock protein beta-6, Heat shock 20 kDa-like protein p20, Heat Shock 20 KDa-Like Protein P20, Hsp20
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
BL28582_Purified_HSPB6_Antibody_1_051319
Western blot of purified anti-HSPB6 antibody (clone BL28582). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced was used as the detection system.
  • BL28582_Purified_HSPB6_Antibody_1_051319
    Western blot of purified anti-HSPB6 antibody (clone BL28582). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced was used as the detection system.
  • BL28582_Purified_HSPB6_Antibody_2_051319
    IHC staining of purified anti-HSPB6 antibody (clone BL28582) on formalin-fixed paraffin-embedded human skeletal muscle tissue. Following antigen retrieval using Sodium Citrate H.I.E.R the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • BL28582_Purified_HSPB6_Antibody_3_051319
    IHC staining of purified anti-HSPB6 antibody (clone BL28582) on formalin-fixed paraffin-embedded human skeletal muscle tissue. Following antigen retrieval using Sodium Citrate H.I.E.R the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • BL28582_Purified_HSPB6_Antibody_4_051319
    IHC staining of purified anti-HSPB6 antibody (clone BL28582) on formalin-fixed paraffin-embedded mouse skeletal muscle tissue. Following antigen retrieval using Sodium Citrate H.I.E.R the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • BL28582_Purified_HSPB6_Antibody_5_051319
    IHC staining of purified anti-HSPB6 antibody (clone BL28582) on formalin-fixed paraffin-embedded rat skeletal muscle tissue. Following antigen retrieval using Sodium Citrate H.I.E.R the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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858201 25 µg 106 CHF
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Description

Heat shock protein B6 (HSPB6), or Hsp20, is a member of the conserved small heat shock protein family, which have important functions in protein folding and aggregation. HSPB6 is ubiquitously expressed, and is particularly enriched in skeletal, cardiac, smooth muscle tissue, and central nervous system. Like many other small heat shock proteins, HSPB6 functions as a molecular chaperone and its expression is up-regulated upon cellular stress conditions to protect cells and tissue from damage. HSPB6 is an important mediator in cardio protective signaling and functions in a BCL-2-associated anthanogene 3 (BAG3)-dependent manner. In multiple sclerosis (MS), HSPB6 level  is markedly increased in astrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810732 (BioLegend Cat. No. 858201)

Antigen Details

Structure
Human HSPB6 is a 117 amino acid protein with a predicted molecular mass of 17 kD and observed molecular mass of 20 kD.
Distribution

Tissue distribution: Ubiquitously expressed.
Cellular distribution: Nucleus, secreted, and cytosol.

Function
HSPB6 is a molecular chaperone that maintains denatured proteins in a folding-competent state.
Interaction
HSPB6 interacts with other sHSPs, BAG3, YWHAZ, PDE4A, and PDE4D.
Cellular targets: denatured proteins
Cell Type
Astrocytes, Neurons
Biology Area
Cardiovascular Biology, Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Heat Shock Proteins
Antigen References
  1. Li F, et al. 2017. Cell Physiol Biochem. 44(1):314-332. 
  2. Fang X, et al. 2019. JCI Insight.  4(4). pii: 126464. 
  3. Peferoen LA, et al. 2015. Acta Neuropathol Commun. 3:87.
Gene ID
126393 View all products for this Gene ID
UniProt
View information about HSPB6 on UniProt.org
Go To Top Version: 1    Revision Date: 05.13.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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