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Purified anti-human ALK Antibody

Pricing & Availability
Clone
W16190A (See other available formats)
Regulatory Status
RUO
Other Names
Anaplastic lymphoma kinase (ALK), ALK tyrosine kinase receptor, cluster of differentiation 246 (CD246), Mutant Anaplastic Lymphoma Kinase, NBLST3
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16190A_PURE_ALK_Antibody_030923
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-human ALK (clone W16190A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • W16190A_PURE_ALK_Antibody_030923
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-human ALK (clone W16190A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
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611551 25 µg 120 CHF
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611552 100 µg 375 CHF
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Description

ALK is expressed widely in the central nervous system, where it plays important roles in neurogenesis and the development of the nervous system. ALK is a receptor tyrosine kinase that phosphorylates tyrosines on target proteins upon ligand binding (known ligands include pleiotrophin, midkine, FAM150A, and FAM150B). ALK was originally discovered in anaplastic large-cell lymphoma cells where a chromosomal translocation leads to constitutive activation of the ALK kinase domain, making ALK a target of inhibition in cancer therapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human ALK
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W16189A is not suitable for ICC, FC, and IHC-P.

W16189A is weakly cross-reactive with mouse ALK in WB.

RRID
AB_2941602 (BioLegend Cat. No. 611551)
AB_2941602 (BioLegend Cat. No. 611552)

Antigen Details

Structure
ALK is a 1,620 amino acid protein with a predicted molecular weight of 176.4 kD.
Distribution

ALK is expressed in the plasma membrane and is expressed in many neuronal subtypes in specific regions of the central and peripheral nervous system.

Function
Upon ligand binding ALK dimerizes and autophosphorylates, gaining kinase activity
Interaction
ALK phosphorylates members of the MAPK and NF-KB signaling pathways
Ligand/Receptor
ALK activation can proceed via binding of pleiotrophin, midkine, FAM150A or FAM150B
Cell Type
Neurons
Biology Area
Cell Proliferation and Viability
Molecular Family
CD Molecules, Protein Kinases/Phosphatase
Antigen References
  1. Souttou B, et al. 2001. J Biol Chem. 276:9526-31.
  2. Hallberg B, et al. 2016. Ann Oncol. 27 Suppl 3:iii4-iii15.
Gene ID
238 View all products for this Gene ID
UniProt
View information about ALK on UniProt.org
Go To Top Version: 1    Revision Date: 03.09.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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