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Purified anti-human Angiopoietin-2 Antibody

Pricing & Availability
Clone
W19419E (See other available formats)
Regulatory Status
RUO
Other Names
Angiopoietin 2, ANG2, Ang-2, ANGPT2
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W19419E_PURE_-Angiopoietin-2_Direct-ELISA_100424
Direct ELISA showing purified anti-human Angiopoietin-2 (clone W19419E) bound to plate-immobilized recombinant human Angiopoietin-2 (Cat. Nos. 753102, 753104, and 753106). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W19419E_PURE_-Angiopoietin-2_Direct-ELISA_100424
    Direct ELISA showing purified anti-human Angiopoietin-2 (clone W19419E) bound to plate-immobilized recombinant human Angiopoietin-2 (Cat. Nos. 753102, 753104, and 753106). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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633752 100 µg 455 CHF
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Description

Angiopoietin-2, also known as ANGPT2, is a member of angiopoietins, which plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of ANGPT2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both ANGPT1 and ANGPT2 are ligands for the receptor tyrosine kinase, Tie-2. ANGPT2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while ANGPT1 predominantly exists as multimers. It has been shown that ANGPT2 is able to promote vascular leakage and neutrophil migration. ANGPT2 can also sensitize TNF-α-induced monocyte adhesion, thus it is considered a proinflammatory factor. Although ANGPT2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of ANGPT2 activate the same survival signaling pathway as ANGPT1 to prevent endothelial cell apoptosis via Tie-2/Akt. Additionally, ANGPT2 was demonstrated to function as an agonist in the absence of ANGPT1, but in its presence, it acts as an antagonist. Human ANGPT2 shares 86% amino acid sequence homology with its murine counterpart.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Recombinant Angiopoietin-2 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19419E) could function as a capture antibody when paired with biotin anti-human Angiopoietin-2 (Cat No. 629453) (clone A15124B) as the detection antibody and using Recombinant Human Angiopoietin-2 (carrier-free) (Cat. Nos. 753102, 753104, and 753106) protein as the assay standard.

Antigen Details

Structure
Oligomer
Distribution

Placenta, uterus, ovaries, and vascular endothelial cells

Function
ANGPT2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
Interaction
Monocytes and endothelial cells
Ligand/Receptor
Tie-2
Cell Type
Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Augustin HG, et al. 2009. Nat Rev Mol Biol. 10:165-77.
  2. Ward NL and Dumont DJ. 2002. Semin Cell Dev Biol. 1:19-27.
  3. Roviezzo F, et al. 2005. J Pharmacol Exp Ther. 314:738-44.
  4. Sturn DH, et al. 2005. Microcirculation.12:393-403.
  5. Murdoch C, et al. 2007. J Immunol. 178:7405-11.
  6. Yuan HT, et al. 2009. Mol Cell Biol. 29:2011-22.
  7. Procopio WN, et al. 1999. J Biol Chem. 274:30196-201.
  8. Bhandari V, et al. 2006. Nature Med. 12:1286-93.
Gene ID
285 View all products for this Gene ID
UniProt
View information about Angiopoietin-2 on UniProt.org
Go To Top Version: 1    Revision Date: 10.04.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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