- Clone
- W19419E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Angiopoietin 2, ANG2, Ang-2, ANGPT2
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
633752 | 100 µg | 455 CHF |
Angiopoietin-2, also known as ANGPT2, is a member of angiopoietins, which plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of ANGPT2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both ANGPT1 and ANGPT2 are ligands for the receptor tyrosine kinase, Tie-2. ANGPT2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while ANGPT1 predominantly exists as multimers. It has been shown that ANGPT2 is able to promote vascular leakage and neutrophil migration. ANGPT2 can also sensitize TNF-α-induced monocyte adhesion, thus it is considered a proinflammatory factor. Although ANGPT2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of ANGPT2 activate the same survival signaling pathway as ANGPT1 to prevent endothelial cell apoptosis via Tie-2/Akt. Additionally, ANGPT2 was demonstrated to function as an agonist in the absence of ANGPT1, but in its presence, it acts as an antagonist. Human ANGPT2 shares 86% amino acid sequence homology with its murine counterpart.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human Recombinant Angiopoietin-2 Protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
Direct ELISA - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19419E) could function as a capture antibody when paired with biotin anti-human Angiopoietin-2 (Cat No. 629453) (clone A15124B) as the detection antibody and using Recombinant Human Angiopoietin-2 (carrier-free) (Cat. Nos. 753102, 753104, and 753106) protein as the assay standard.
Antigen Details
- Structure
- Oligomer
- Distribution
-
Placenta, uterus, ovaries, and vascular endothelial cells
- Function
- ANGPT2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
- Interaction
- Monocytes and endothelial cells
- Ligand/Receptor
- Tie-2
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Augustin HG, et al. 2009. Nat Rev Mol Biol. 10:165-77.
- Ward NL and Dumont DJ. 2002. Semin Cell Dev Biol. 1:19-27.
- Roviezzo F, et al. 2005. J Pharmacol Exp Ther. 314:738-44.
- Sturn DH, et al. 2005. Microcirculation.12:393-403.
- Murdoch C, et al. 2007. J Immunol. 178:7405-11.
- Yuan HT, et al. 2009. Mol Cell Biol. 29:2011-22.
- Procopio WN, et al. 1999. J Biol Chem. 274:30196-201.
- Bhandari V, et al. 2006. Nature Med. 12:1286-93.
- Gene ID
- 285 View all products for this Gene ID
- UniProt
- View information about Angiopoietin-2 on UniProt.org
Other Formats
View All Angiopoietin-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Angiopoietin-2 | W19419E | Direct ELISA |
Compare Data Across All Formats
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Purified anti-human Angiopoietin-2
Direct ELISA showing purified anti-human Angiopoietin-2 (clo...
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