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Purified anti-human APCDD1 (DRAPC1) Antibody

Pricing & Availability
Clone
7.13 (See other available formats)
Regulatory Status
RUO
Other Names
Adenomatosis polyposis coli down-regulated 1, B7323, DRAPC1, P7019
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
7.13_PURE_APCDD1_Antibody_FC_1_051815
Human colon adenocarcinoma cell line (SW480) cells were stained with purified APCDD1 (clone 7.13) or mouse IgG2a, κ isotype control followed by anti-mouse IgG-PE.
  • 7.13_PURE_APCDD1_Antibody_FC_1_051815
    Human colon adenocarcinoma cell line (SW480) cells were stained with purified APCDD1 (clone 7.13) or mouse IgG2a, κ isotype control followed by anti-mouse IgG-PE.
  • 7.13_PURE_APCDD1_Antibody_IF_2_051815
    Human colon adenocarcinoma cell line (SW480) cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml purified anti-human APCDD1/DRAPC1 (clone 7.13) (red) at room temperature for three hours in the dark followed by 2.5 µg/ml DyLight™ 594 anti-mouse IgG staining for two hours at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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367302 100 µg 265 CHF
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Description

APCDD1 (adenomatosis polyposis coli down-regulated 1), also known as B7323, DRAPC1, or FP7019, is a 514 amino acid single-pass type I membrane protein. APCDD1 is expressed in the gastrointestinal tract, placental trophoblasts, and both epidermal and dermal compartments from human hair follicles. It is also weakly expressed in lymphoid tissues. APCDD1 has been reported to be directly regulated by the beta-catenin/ITF-2 complex and that its elevated expression promotes the proliferation of colonic epithelial cells. It is thought to function as a developmental target of the beta-catenin pathway and may play an important role in colorectal tumorigenesis. Recently, it was found to negatively regulate Wnt signaling in a cell-autonomous manner.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human APCDD1-His.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration of 10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565749 (BioLegend Cat. No. 367302)

Antigen Details

Structure
514 amino acid single-pass type I membrane protein.
Distribution

Gastrointestinal track and placental trophoblastis, epidermal and dermal compartments of the hair follicle, and weak expression in lymphoid tissues.

Function
May regulate a diversity of biological process controlled by Wnt signaling, regulated by beta-catenin/ITF-2 complex.
Interaction
Plays an important role in colorectal tumorigenesis.
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Shimomura Y, et al. 2010. Nature 7291:1043.
2. Takahashi M, et al. 2002. Cancer Res. 20:5651.

Gene ID
147495 View all products for this Gene ID
UniProt
View information about APCDD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.30.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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