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- Clone
- S21005B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ARK, AXL receptor tyrosine kinase, AXL transforming sequence/gene, AXL oncogene, JTK11, Tyro7, tyrosine-protein kinase receptor UFO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were stained with purified anti-human AXL (clone S21005B) (left) or purified mouse IgG1 κ isotype control (right), followed by anti-mouse IgG PE. Cells were then co-stained for lineage of human CD3/CD19/CD14/CD15 FITC and CD303 APC. Data shown was gated lineage negative population. -
Purified anti-human AXL (clone S21005B) blocks GAS6 induced AKT phosphorylation. H1299 cells were incubated with 10 µg Ultra-LEAF™ anti-human AXL (clone S21005B) (filled histogram) or 10 µg Ultra-LEAF™ mouse IgG1, κ isotype control (open histogram) at 37°C for 60 minutes, then 50 nM (final concentration) recombinant human GAS6 was added to induce phosphorylation. Cells were then fixed by adding an equal volume of Fixation Buffer (Cat. No. 420801), permeabilized by adding True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-Phospho-AKT1 (Ser473) APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 386202 | 100 µg | 235 CHF | ||||
AXL, also known as ARK or UFO, is a widely expressed glycoprotein in the TAM (Tyro3/Dtk, AXL, and Mer) receptor tyrosine kinase family. AXL is highly expressed in solid cancers. It is also expressed on a small population of human dendritic cells that share characteristics of classical and plasmacytoid dendritic cells. AXL binds the vitamin K-dependent growth arrest specific 6 (Gas6) protein and induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. AXL is also involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have also shown that AXL and Mer are both linked to Alzheimer's disease
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC- Quality tested
Block - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone S21005B recognizes a different epitope compared to clone S21005C.
Clone S21005B can block GAS6 induced AKT and ERK phosphorylation in H1299 cells. - RRID
-
AB_2941567 (BioLegend Cat. No. 386202)
Antigen Details
- Structure
- Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain
- Distribution
-
Highly expressed in solid cancers and also expressed on human blood plasmacytoid dendritic cells and myeloid dendritic cells
- Function
- Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. It is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have also shown that AXL and Mer are both linked to Alzheimer's disease.
- Ligand/Receptor
- Gas6 and Protein S
- Cell Type
- Dendritic cells, NK cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Death, Cell Proliferation and Viability, Immuno-Oncology, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, Cytokines/Chemokines
- Antigen References
-
- Holland S, et al. 2005. Canc Res. 65:9294-303.
- Lee WP, et al. 2002. Oncogene. 21:329-36.
- Demarchi F, et al. 2001. J Biol Chem. 276:31738-44.
- Gene ID
- 558 View all products for this Gene ID
- UniProt
- View information about AXL on UniProt.org
Other Formats
View All AXL Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human AXL | S21005B | FC,Block |
| PE anti-human AXL | S21005B | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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