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Purified anti-human Bcl11b Antibody

Pricing & Availability
Clone
W17189A (See other available formats)
Regulatory Status
RUO
Other Names
CTIP-2, CTIP2, hRIT1-alpha, SMARCM2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W17189A_PURIFIED_Bcl11b_Antibody_1_033023
Human peripheral blood mononuclear cells were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) then stained with purified anti-human Bcl11b (clone W17189A) (left) or purified rat IgG2a, κ isotype control (right) antibody, followed by anti-rat IgG PE. Cells were stained for anti-human CD3 APC. Data was gated on lymphocyte populations.
  • a. W17189A_PURIFIED_Bcl11b_Antibody_1_033023
    Human peripheral blood mononuclear cells were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) then stained with purified anti-human Bcl11b (clone W17189A) (left) or purified rat IgG2a, κ isotype control (right) antibody, followed by anti-rat IgG PE. Cells were stained for anti-human CD3 APC. Data was gated on lymphocyte populations.
  • b. W17189A_PURIFIED_Bcl11b_Antibody_2_ICFC_033023
    Jurkat cells were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) then stained with purified anti-human Bcl11b (clone W17189A) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • c. W17189A_PURE_Bcl11b_Antibody_WB_051223
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-human Bcl11b (clone W17189A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • d. W17189A_PURIFIED_Bcl11b_Antibody_ICH-P_033023
    IHC staining using purified anti-human Bcl11b (clone W17189A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the antibody (left) or left untreated (right) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 µM
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384502 100 µg 422 CHF
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Description

The transcription factor, Bcl11b, is expressed in the thymus, mainly T cells. It is indispensable for T lineage development. When Bcl11b was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro, and effectively prevented tumor metastasis in vivo. Therefore, ITNKs may represent a new cell source for cell-based therapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
RP 1-250 (generated by E. coli)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
WB, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941561 (BioLegend Cat. No. 384502)

Antigen Details

Structure
894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution

Nucleus

Function
Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction
Interacts with TFCOUP1, SIRT1, ARP1, and EAR2
Molecular Family
Nuclear Markers
Antigen References
  1. Liu P, et al. 2011. Immunol Rev. 238:138-49.
  2. Ikawa T, et al. 2010. Science. 329:93-6.
  3. Li L, et al. 2010. Science. 329:89-93.
  4. Li P, et al. 2010. Science. 329:85-9.
  5. Tydell CC, et al. 2007. J Immunol. 179:4238.
  6. Avram D, et al. 2002. Biochem J. 368:555-63.
Gene ID
19882 View all products for this Gene ID
UniProt
View information about Bcl11b on UniProt.org
Go To Top Version: 1    Revision Date: 03.24.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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