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Purified anti-human BMI1 Antibody

Pricing & Availability
Clone
W20031H (See other available formats)
Regulatory Status
RUO
Other Names
Polycomb complex protein BMI-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_W20031H_PURE_BMI1_Antibody_071222
Human peripheral blood leukocytes were stained with anti-human CD15 PerCP/Cyanine 5.5, fixed and intracellularly stained with purified anti-human BMI1 (clone W20031H) (left) or rat IgG2a, κ isotype control followed by PE anti-rat IgG (right) using the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Data shown are gated on CD45-positive cells.
  • A_W20031H_PURE_BMI1_Antibody_071222
    Human peripheral blood leukocytes were stained with anti-human CD15 PerCP/Cyanine 5.5, fixed and intracellularly stained with purified anti-human BMI1 (clone W20031H) (left) or rat IgG2a, κ isotype control followed by PE anti-rat IgG (right) using the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Data shown are gated on CD45-positive cells.
  • B_W20031H_PURE_BMI1_Antibody_2_071222
    HeLa cells were fixed with 4% paraformaldehyde for 30 minutes, permeabilized with 0.5% Triton X-100 for 15 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL purified anti-human BMI1 (clone W20031H) overnight at 4°C followed by Alexa Fluor® 555 goat anti-rat IgG (red) for one hour at room temperature. Cells were counterstained with Flash Phalloidin Green 488 (green).
  • C_W20031H_PURE_BMI1_Antibody_072522
    15 µg total cell lysate from U937 cells (lane1), HEL cells (lane 2), NTERA-2 (lane 3), HeLa cells (lane 4,), were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a PVDF membrane, and probed with 1 µg/mL purified anti-human BMI1 (clone W20031H). Proteins were visualized using an HRP goat anti-rat IgG (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-GAPDH was used as a loading control.
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381402 100 µg 314 CHF
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Description

BMI1 is a ring finger protein that is a major component of the polycomb group complex 1 (PRC1). Acting via chromatin remodeling and modification of histones, PRC1 maintains the transcriptionally repressive state of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. BMI1 is essential for the self-renewal of hematopoietic stem cells and neuronal stem cells, in part via repression of genes involved in senescence. Aberrant expression of BMI1 is associated with many cancers and resistance to chemotherapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human BMI1 protein, full length
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927847 (BioLegend Cat. No. 381402)

Antigen Details

Structure
Zing finger, Ring-type
Distribution

Nucleus, cytoplasm

Function
Chromatin regulator, repressor
Interaction
RNF2, UB2D3, PHC2, E4F1
Ligand/Receptor
Metal-binding, Zinc
Cell Type
Embryonic Stem Cells, Lymphocytes, T cells
Biology Area
Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Satijn DP, et al. 1997. Mol Cell Biol. 17:105–13.
  2. Park IK, et al. 2003. Nature. 423:302–5.
  3. Shakhova O, et al. 2005. J Mol Med (Berl). 83:596–600.
  4. Lessard J, et al. 2003. Nature. 423:255–60.
  5. Abdouh M, et al. 2009. J Neurosci. 29:8884–96.
Gene ID
648 View all products for this Gene ID
UniProt
View information about BMI1 on UniProt.org
Go To Top Version: 1    Revision Date: 07.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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