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Purified anti-human CCL1 (I-309) Antibody

Pricing & Availability
Clone
A16001E (See other available formats)
Regulatory Status
RUO
Other Names
T lymphocyte-secreted protein I-309
Isotype
Mouse IgG1, κ
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Product Citations
publications
A16001E_PURE_CCL1_Antibody_050923
Direct ELISA showing purified anti-human CCL1 (I-309) (clone A16001E) bound to plate-immobilized recombinant human CCL1 (I-309) protein (Cat. Nos. 582702, 582704, 582706 and 582708). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A16001E_PURE_CCL1_Antibody_050923
    Direct ELISA showing purified anti-human CCL1 (I-309) (clone A16001E) bound to plate-immobilized recombinant human CCL1 (I-309) protein (Cat. Nos. 582702, 582704, 582706 and 582708). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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613252 100 µg 235 CHF
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Description

CCL1 was initially identified as a secreted protein derived from activated T cells. The CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation. As a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatic patients compared with normal controls. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro, and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant CCL1 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A16001E) could function as a capture antibody when paired with Biotin anti-human CCL1 antibody (Cat. No. 612253) (clone A16001C) as the detection antibody and using Recombinant Human CCL1 (I-309) (carrier-free) protein (Cat. Nos. 582702, 582704, 582706 and 582708) as the assay standard.

RRID
AB_2941611 (BioLegend Cat. No. 613252)

Antigen Details

Structure
Chemokine
Distribution

T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells

Function
CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity.
Interaction
T cells, Th2 cells, macrophages, microglia, FOXP3+ CD4 regulatory T cells, skin-homing CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells
Ligand/Receptor
CCR8 (CDw198)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Miller MD, et al. 1989. J Immunol. 143:2907-16.
  2. Haque NS, et al. 2004. Blood. 103:1296-304.
  3. Gombert M, et al. 2005. J Immunol. 174:5082-91.
  4. Montes-Vizuet R, et al. 2006. Eur Respir J. 28:59-67.
  5. Soler D, et al. 2006. J Immunol. 177:6940-51.
  6. Gonzalo JA, et al. 2007. J Immunol. 179:1740-50.
  7. Mutalithas K, et al. 2010. Clin Exp Allergy. 40:1175-85.
Gene ID
6346 View all products for this Gene ID
UniProt
View information about CCL1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.10.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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