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Purified anti-human CCL5 (RANTES) Antibody

Pricing & Availability
Clone
J047C5 (See other available formats)
Regulatory Status
RUO
Other Names
SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine A5
Isotype
Mouse IgG2b, κ
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Product Citations
publications
CCL5_Human_Antibody_ELISA_122214
  • CCL5_Human_Antibody_ELISA_122214
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526402 500 µg 347 CHF
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Description

CCL5 (RANTES), also known as CCL5, is a 7.8 kD member of the CC family of chemokines, which is expressed in T cells and monocytes/macrophages. RANTES is strongly chemotactic for monocytes/macrophages, memory T cells, eosinophils, and induces histamine release from basophils. In a solution, RANTES can form high molecular weight aggregates that exhibit dissimilar biological activities to its dimeric form. The aggregates cause T cell and neutrophil activation while promoting HIV infection, whereas the dimeric form exhibits RANTES-induced chemotaxis and inhibits HIV infection. As T cells become activated, RANTES secretion appears to decrease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL5 recombinant protein ranging from 500 to 7.8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified J047C5 (Cat. No. 526402) antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5197 antibody (Cat. No. 519703) as the detection antibody and Recombinant Human CCL5 (RANTES) (ELISA Std.) (Cat. No. 580209) as the standard.

Note: For testing human CCL5 (RANTES) in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 440807 & 440808) are specially developed and recommended.

RRID
AB_2563996 (BioLegend Cat. No. 526402)

Antigen Details

Distribution
RANTES/CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. RANTES is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C.
Function
RANTES can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. RANTES modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by RANTES.
Interaction
RANTES/CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
Ligand/Receptor
CCR1, CCR3, CCR4, and CCR5.
Cell Type
Lymphocytes, Macrophages, Eosinophils, Platelets, Fibroblasts
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Brown KD, et al. 1989. J. Immunol. 142:679.
2. Schall TJ, et al. 1990. Nature 6294:669-671.
3. Cocchi F, et al. 1995. Science 270:1811-1815.
4. Shahrara S, et al. 2006. J. Immunol. 177:5077-5087.
5. Larrubia JR, et al. 2008. World J. Gastroenterol. 14:7149-7159.
6. Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920-1927.
7. Von Luettichau, et al. 1996. Cytokine 8:89.
8. Schall TJ. 1991. Cytokine 3:165.
9. Marques RE, et al. 2013. Expert Opin. Ther. Targets 17:1439.

Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org
Go To Top Version: 1    Revision Date: 12.30.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human CCL5 (RANTES)

    CCL5_Human_Antibody_ELISA_122214
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