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Purified anti-human CCL5 (RANTES) Antibody

Pricing & Availability
Clone
Poly5197 (See other available formats)
Regulatory Status
RUO
Other Names
SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine A5
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5197_Pure_CCL5_Antibody_022024
Direct ELISA showing purified anti-human CCL5 (clone Poly5197) bound to plate-immobilized recombinant human CCL5 (RANTES) (Cat. Nos. 580202, 580204, and 580206). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Anti-Goat IgG. TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • Poly5197_Pure_CCL5_Antibody_022024
    Direct ELISA showing purified anti-human CCL5 (clone Poly5197) bound to plate-immobilized recombinant human CCL5 (RANTES) (Cat. Nos. 580202, 580204, and 580206). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Anti-Goat IgG. TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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519702 100 µg 420 CHF
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Description

CCL5 (RANTES), also known as CCL5, is a 7.8 kD member of the CC family of chemokines, which is expressed in T cells and monocytes/macrophages. RANTES is strongly chemotactic for monocytes/macrophages, memory T cells, eosinophils, and induces histamine release from basophils. In a solution, RANTES can form high molecular weight aggregates that exhibit dissimilar biological activities to its dimeric form. The aggregates cause T cell and neutrophil activation while promoting HIV infection, whereas the dimeric form exhibits RANTES-induced chemotaxis and inhibits HIV infection. As T cells become activated, RANTES secretion appears to decrease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human CCL5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Application Notes

ELISA Detection: The biotinylated Poly5197 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified  antibody J047C5 (Cat. No. 526402) as the capture antibody and recombinant human CCL5 (RANTES) (Cat. No. 580209) as the standard.

Note: For testing human CCL5 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human CCL5 (RANTES) ELISA Kit with Pre-coated Plates (Cat. No. 440807 & 440808) are specially developed and recommended.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly5197) could function as a detection antibody when paired with purified anti-human CCL5 (RANTES) (Cat No. 620252) (clone A19042E) as the capture antibody and using Recombinant Human CCL5 (RANTES) (carrier-free) protein (Cat. Nos. 580202, 580204, and 580206) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_3106012 (BioLegend Cat. No. 519702)

Antigen Details

Distribution

RANTES/CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. RANTES is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C.

Function
RANTES can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. RANTES modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by RANTES.
Interaction
RANTES/CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
Ligand/Receptor
CCR1, CCR3, CCR4, and CCR5.
Cell Type
Eosinophils, Fibroblasts, Lymphocytes, Macrophages, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Brown KD, et al. 1989. J. Immunol. 142:679.
2. Schall TJ, et al. 1990. Nature 6294:669-671.
3. Cocchi F, et al. 1995. Science 270:1811-1815.
4. Shahrara S, et al. 2006. J. Immunol. 177:5077-5087.
5. Larrubia JR, et al. 2008. World J. Gastroenterol. 14:7149-7159.
6. Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920-1927.
7. Von Luettichau, et al. 1996. Cytokine 8:89.
8. Schall TJ. 1991. Cytokine. 3:165.
9. Marques RE, et al. 2013. Expert Opin. Ther. Targets 17:1439.

Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org
Go To Top Version: 1    Revision Date: 02.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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