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Purified anti-human CCL7 (MCP-3) Antibody

Pricing & Availability
Clone
A15027A (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemotactic protein 3 (MCP-3), Small inducible cytokine subfamily A. member
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15027A_PURE_-CCL7-_Direct-ELISA_Antibody
Direct ELISA showing purified anti-human CCL7 (MCP-3) (clone A15027A) bound to plate-immobilized recombinant human CCL7 (MCP-3). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A15027A_PURE_-CCL7-_Direct-ELISA_Antibody
    Direct ELISA showing purified anti-human CCL7 (MCP-3) (clone A15027A) bound to plate-immobilized recombinant human CCL7 (MCP-3). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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630652 100 µg 415 CHF
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Description

CCL7, also known as MCP-3, was initially purified from cytokine-stimulated human osteosarcoma cell line (MG-63) conditioned medium. CCL7 has high similarity with CCL2/MCP-1 (71%), CCL8/MCP-2 (58%), and CCL13/MCP-4 (59%) at the amino acid level. Chemokines play a key role in inflammation and, in the initial phase, CXC chemokines chemoattract polymorphonuclear leukocytes (PMNs). The next step in the inflammatory processes is directed in part by CC chemokines, including the monocyte chemoattractant proteins (CCL2, CCL7, CCL8, and CCL13) that chemoattract additional leukocytes subsets. It has been reported that matrix metalloproteinases (MMPs) regulate chemokine bioactivity, increasing or decreasing their chemoattractant property. MMP-8 and MMP-9 modulate CXC chemokines, and MMP-2 truncates the N-terminal of CCL7, eliminating the first four amino acids producing CCL7 (5-76 residues), which leads to a loss of activity. The truncated CCL7 binds to its receptor, but it does not induce signaling transduction. Monocyte chemoattractant proteins (MCPs) are truncated by different MMPs to create potent antagonists and induce decreases in inflammation. For example, MMP-13 cleaves CCL2 and CCL7 and generates potent receptor antagonists. Also, CCL7 and CCL2 play an important role in the pathogenesis of systemic sclerosis (SSc). It has been described that they are overexpressed in SSc and activate collagen production in dermal fibroblasts. In addition, CCL7 and CCL21 are highly expressed in gastric cancer and are associated with lymph node metastasis and poor prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant CCL7 (MCP3) Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15027A) could function as a detection antibody when paired with purified anti-human CCL7 antibody (Cat No. 630552) (clone A15027F) as the capture antibody and using Recombinant Human CCL7 (MCP-3) (carrier-free) protein as the assay standard. For assay development flexibility purposes, this product is provided as a purified format. 

RRID
AB_3662345 (BioLegend Cat. No. 630652)

Antigen Details

Structure
Chemokine
Distribution

Fibroblasts, mast cells, astrocytes, endothelial cells, monocytes, and macrophages

Function
CCL7 chemoattracts monocytes, T cells, NK cells, eosinophils, and basophils. CCL7 protein is secreted by mononuclear leukocytes after stimulation with IL1β, IFN-α, IFN-β, measles virus, poly I-C, or Con A. In fibroblasts, CCL7 mRNA and protein are synergistically induced after treatment with IL-1β and IFN-γ. CCL7 is stimulated in murine fibroblasts and astrocytes by TGF-β and TNF-α respectively. Anti-inflammatory cytokines (IL-4, IL-10, or IL-13) inhibit the expression of CCL7 in monocytes.
Interaction
Monocytes, T cells, NK cells, dendritic cells, mast cells, eosinophils, and basophils
Ligand/Receptor
CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).
Cell Type
Embryonic Stem Cells
Biology Area
Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
Antigen References
  1. Van Damme J, et al. 1992. J Exp Med. 176:59-65.
  2. Menten P, et al. 1999. Eur J Immunol. 29:678-85.
  3. McQuibban GA, et al. 2000. Science. 289:1202-6.
  4. Menten P, et al. 2001. Eur Cytokine Netw. 12:554-60.
  5. Dean RA, et al. 2008. Blood. 112:3455-64.
  6. Renner NA, et al. 2011. J Neurovirol. 17:146-52.
  7. Hwang TL, et al. 2012. World J Gastroenterol. 18:1249-56.
  8. Feuser K, et al. 2012. Cytokine. 58:178-85.
Gene ID
6354 View all products for this Gene ID
UniProt
View information about CCL7 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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