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Purified anti-human CCR10 Antibody

Pricing & Availability
Clone
6588-5 (See other available formats)
Regulatory Status
RUO
Other Names
GPR-2, C-C chemokine receptor 10
Isotype
Armenian Hamster IgG
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Product Citations
publications
A_6588-5_PE_050109.jpg
Human peripheral blood lymphocytes stained with CD3 APC and purified 6588-5 conjugated with PE
  • A_6588-5_PE_050109.jpg
    Human peripheral blood lymphocytes stained with CD3 APC and purified 6588-5 conjugated with PE
  • B_6588-5_PE_050109.jpg
    Human peripheral blood lymphocytes stained with CLA (HECA-452) FITC and purified 6588-5 conjugated with PE (dot plot analysis is derived from CD3+ cell population)
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341502 100 µg 229 CHF
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Description

CCR10, also known as GPR-2, is a G-protein-coupled seven transmembrane CC-chemokine receptor. It is the receptor of CCL27 (CTACK/ALP/ILC/ESkine) and CCL28 (MEC) and is expressed on a small subset of T memory cells, IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts. The interaction of CCR10 with its ligands plays a role in the regulation of T cell homing into cutaneous site and IgA-secreting cells migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
N-terminal peptide of human CCR10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

It has been observed that clone 6588-5 can interact with some tandem-dye antibody conjugates during multi-color staining, potentially leading to unwanted staining. These dyes include PE/Cyanine7, PE/Cyanine5, PE/Dazzle™ 594, APC/Cyanine7, APC/Fire™ 750, and PerCP/Cyanine5.5.  This interaction can be resolved by sequentially staining with the 6588-5 antibody first and then followed by other antibodies of interest.

Product Citations
  1. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
RRID
AB_1595419 (BioLegend Cat. No. 341502)

Antigen Details

Structure
G-protein-coupled seven transmembrane protein
Distribution

Small subsets of T memory cells and IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts

Function
T cell homing into cutaneous site, regulation of IgA-secreting cells migration
Ligand/Receptor
CCL27 (CTACK/ALP/ILC/ESkine), CCL28 (MEC)
Cell Type
B cells, Endothelial cells, Fibroblasts, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Hudak S, et al. 2002. J. Immunol. 169:1189
2. Kunkel EJ, et al. 2003. J. Clin. Invest. 111:1001
3. Homey B, et al. 2002. Nature Medicine. 8:157
4. Nakayama T, et al. 2002. J. Virol. 76:3072

Gene ID
2826 View all products for this Gene ID
UniProt
View information about CCR10 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 5    Revision Date: 09.16.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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