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Purified anti-human CD112R (PVRIG) Antibody

Pricing & Availability
Clone
W16216D (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus receptor-related immunoglobulin domain containing protein, PVRIG, C7orf15, CD112 receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16216D_PURE_CD112R_Antibody_050520
Jurkat cells were infected with control shRNA (open purple histograms) or CD112R-specific shRNA (filled purple histogram) letiviral particles. These cells were stained with purified CD112R (PVRIG) (clone W16216D); the control shRNA lentivirus infected cells were also stainined with purified rat IgG2a, κ isotype control (black dashed open histogram). The binding of antibodies was detected by goat anti-rat IgG APC.
  • W16216D_PURE_CD112R_Antibody_050520
    Jurkat cells were infected with control shRNA (open purple histograms) or CD112R-specific shRNA (filled purple histogram) letiviral particles. These cells were stained with purified CD112R (PVRIG) (clone W16216D); the control shRNA lentivirus infected cells were also stainined with purified rat IgG2a, κ isotype control (black dashed open histogram). The binding of antibodies was detected by goat anti-rat IgG APC.
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301502 100 µg 329 CHF
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Description

CD112R gene encodes a single transmembrane protein consisting of a single extracellular IgV domain. Its long intracellular domain contains two tyrosine residues, one of which is within an ITIM-like motif. CD112R was initially named PVRIG for the homology observed between its second exon and the variable immunoglobulin domain of the polio virus receptor (PVR/CD155) and polio virus receptor-like (PVRL) genes. CD112R is a cell surface receptor for CD112/Nectin-2, it compete against CD226 in binding to CD112. Following interaction with CD112, CD112R inhibits T-cell proliferation and the disruption of interaction between CD112R and CD112 enhances T cell response. Published studies suggest that CD112R is a novel co-inhibitory receptor, or a checkpoint molecule, that suppressed TCR medicated signal.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD112R transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2860764 (BioLegend Cat. No. 301502)

Antigen Details

Structure
IgV domain; ITIM motif
Distribution

T cells, NK cells

Function
Cell surface receptor for CD112, negative regulator of T cell response
Ligand/Receptor
CD112 (Nectin-2)
Cell Type
Leukocytes, Lymphocytes, NK cells, T cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
Immune Checkpoint Receptors
Antigen References
  1. Xu F, et al. 2017. Cancer Immunol. Immunother. 66:1367-1375.
  2. Zhu Y, et al. 2016. J. Exp. Med. 213(2):167-76.
Gene ID
79037 View all products for this Gene ID
UniProt
View information about CD112R on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.05.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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