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Purified anti-human CD116 Antibody

Pricing & Availability
Clone
4H1 (See other available formats)
Regulatory Status
RUO
Other Names
GM-CSFRα chain
Isotype
Mouse IgG1, κ
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Product Citations
publications
4H1
Human peripheral blood monocytes stained with 4H1 FITC
  • 4H1
    Human peripheral blood monocytes stained with 4H1 FITC
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305902 100 µg 101 CHF
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Description

CD116 is a 70-85 kD α chain of the GM-CSF receptor. It combines with CDw131 β chain to form the high affinity GM-CSF receptor. A soluble form of CD116, which binds GM-CSF with a relatively low affinity, has been identified. In addition, an alternatively spliced form of CD116 with an altered cytoplasmic tail has been described. CD116 is expressed on various myeloid cells including monocytes, macrophages, neutrophils, eosinophils, dendritic cells and their precursors, fibroblasts, and endothelial cells. CD116 is expressed on myeloid leukemias, osteogenic sarcoma cell lines, osteoblast-like cells and breast and lung carcinoma cell lines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human GM-CSFRα transfected COS cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting1, and immunohistochemical staining of acetone-fixed frozen tissue sections4 and paraffin-embedded tissue sections5.

Application References

(PubMed link indicates BioLegend citation)
  1. Stomski FC, et al. 1998. J. Biol. Chem. 273:1192. (WB)
  2. McClure B, et al. 2001. Blood 98:3165. (IP)
  3. Guthridge MA, et al. 2004. Blood 103:820.
  4. Xiong S, et al. 2013. J. Clin. Invest. 123:4264. (IHC)
  5. Sawada H, et al. 2014. J. Exp. Med. 211:263. (IHC).
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  2. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  3. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  4. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  5. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  6. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  7. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  8. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  9. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  10. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314568 (BioLegend Cat. No. 305902)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70-85 kD
Distribution

Monocytes, granulocytes, dendritic cells, endothelial precursors

Function
Myeloid hematopoietic cell proliferation, differentiation
Ligand/Receptor
GM-CSF
Cell Type
Dendritic cells, Endothelial cells, Granulocytes, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
1438 View all products for this Gene ID
UniProt
View information about CD116 on UniProt.org
Go To Top Version: 3    Revision Date: 08.22.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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